Antioxidant defenses influence HIV-1 replication and associated cytopathic effects

Paul A. Sandstrom, Judith Murray, Thomas M. Folks, Alan M. Diamond

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

HIV-infected cells often exhibit reduced levels of antioxidant enzymes and thiols. To investigate the role of cellular antioxidant defenses in the progression of an acutely spreading HIV-1 infection, human Sup-T1 T cells were engineered to overexpress the selenium-dependent glutathione peroxidase, GSHPx-1. This enzyme represents a major cellular defense mechanism against toxicity associated with reactive oxygen species (ROS). T cells engineered to produce elevated GSHPx-1 activity displayed accelerated viral replication and associated cytopathic effects compared to control cells. Conversely, the inhibition of the synthesis of glutathione with buthione sulfoximine (BSO) resulted in the attenuation of viral replication in Sup-T1 cells. Similarly, exposure of human peripheral blood lymphocytes (PBLs) to low, nontoxic levels of BSO resulted in an approximately 80% decline in HIV-1 replication as indicated by Western blot analysis of viral proteins.

Original languageEnglish (US)
Pages (from-to)1485-1491
Number of pages7
JournalFree Radical Biology and Medicine
Volume24
Issue number9
DOIs
StatePublished - Jun 1998
Externally publishedYes

Fingerprint

T-cells
HIV-1
Antioxidants
Lymphocytes
Viral Proteins
Enzymes
Selenium
Sulfhydryl Compounds
Glutathione
T-Lymphocytes
Toxicity
Reactive Oxygen Species
Blood
HIV Infections
Western Blotting
HIV
glutathione peroxidase GPX1

Keywords

  • BSO
  • Free radical
  • Glutathione
  • Glutathione peroxidase
  • HIV-1
  • Selenium

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Antioxidant defenses influence HIV-1 replication and associated cytopathic effects. / Sandstrom, Paul A.; Murray, Judith; Folks, Thomas M.; Diamond, Alan M.

In: Free Radical Biology and Medicine, Vol. 24, No. 9, 06.1998, p. 1485-1491.

Research output: Contribution to journalArticle

Sandstrom, Paul A. ; Murray, Judith ; Folks, Thomas M. ; Diamond, Alan M. / Antioxidant defenses influence HIV-1 replication and associated cytopathic effects. In: Free Radical Biology and Medicine. 1998 ; Vol. 24, No. 9. pp. 1485-1491.
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