Antigenic structure of the influenza B virus hemagglutinin: Nucleotide sequence analysis of antigenic variants selected with monoclonal antibodies

Michael T Berton, C. W. Naeve, R. G. Webster

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

We report here the complete nucleotide sequence of the hemagglutinin (HA) gene of influenza B virus B/Oregon/5/80 and, through comparative sequence analysis, identify amino acid substitutions in the HA1 polypeptide responsible for the antigenic alterations in laboratory-selected antigenic variants of this virus. The complete nucleotide sequence of the B/Oregon/5/80 HA gene was established by a combination of chemical sequencing of a full-length cDNA clone and dideoxy sequencing of the virion RNA. The nucleotide sequence is very similar to previously reported influenza B virus HA gene sequences and differs at only nine nucleotide positions from the B/Singapore/222/79 HA gene. The nucleotide sequences of the HA1 portions of the HA genes of 18 laboratory-selected antigenic variants were determined by the dideoxy method. Comparison of the deduced amino acid sequences of the parental and variant HA1 polypeptides revealed 16 different amino acid substitutions at nine positions. All amino acid substitutions resulted from single-point mutations, and no double mutants were detected, demonstrating that as in the influenza A viruses, single amino acid substitutions are sufficient to alter the antigenicity of the HA molecule. Many of the amino acid substitutions in the variants occurred at positions also observed to change in natural drift strains. The substitutions appear to identify at least two immunodominant regions which correspond to proposed antigenic sites A and B on the influenza A virus H3 HA.

Original languageEnglish (US)
Pages (from-to)919-927
Number of pages9
JournalJournal of Virology
Volume52
Issue number3
StatePublished - 1984
Externally publishedYes

Fingerprint

Influenza B virus
Hemagglutinins
hemagglutinins
Sequence Analysis
monoclonal antibodies
sequence analysis
amino acid substitution
Amino Acid Substitution
Monoclonal Antibodies
nucleotide sequences
Genes
Influenza A virus
polypeptides
genes
RNA Sequence Analysis
Immunodominant Epitopes
Peptides
Singapore
point mutation
virion

ASJC Scopus subject areas

  • Immunology

Cite this

Antigenic structure of the influenza B virus hemagglutinin : Nucleotide sequence analysis of antigenic variants selected with monoclonal antibodies. / Berton, Michael T; Naeve, C. W.; Webster, R. G.

In: Journal of Virology, Vol. 52, No. 3, 1984, p. 919-927.

Research output: Contribution to journalArticle

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