Antigenic determinants of the chlamydial major outer membrane protein resolved at a single amino acid level

Guangming Zhong, R. C. Brunham

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Antigenic determinants were identified from seven chlamydial major outer membrane proteins by using overlapping hexapeptides and polyclonal antisera. Sixty-one determinants were detected, and 30 were surface exposed on the native organisms. The two negatively charged residues, aspartic acid and glutamic acid, were found most often in determinants. Thirteen antigenic sites were further characterized by alanine substitution. Differences in fine specificitites of these linear determinants were observed in alanine substitution profiles. Five determinants had adjacent critical residues, while eight had critical residues alternated with noncritical residues. Complete replacement analysis of two antigenic determinants provided more detailed information for elucidating the structural basis of the specificity of antigen-antibody interaction and suggested a correlation between sequence conservation and tolerance to amino acid substitution for antigenic sites subject to intense immune selection pressure.

Original languageEnglish (US)
Pages (from-to)1141-1147
Number of pages7
JournalInfection and Immunity
Volume59
Issue number3
StatePublished - 1991
Externally publishedYes

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Alanine
Epitopes
Membrane Proteins
Amino Acids
Antibody Specificity
Amino Acid Substitution
Aspartic Acid
Immune Sera
Glutamic Acid
Antigens
Pressure

ASJC Scopus subject areas

  • Immunology

Cite this

Antigenic determinants of the chlamydial major outer membrane protein resolved at a single amino acid level. / Zhong, Guangming; Brunham, R. C.

In: Infection and Immunity, Vol. 59, No. 3, 1991, p. 1141-1147.

Research output: Contribution to journalArticle

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