Anticonvulsant drug actions on in vitro and in vivo levels of cyclic AMP in the mouse brain

G. C. Palmer, D. J. Jones, M. A. Medina, W. B. Stavinoha

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Injections of pentylenetetrazol to mice produced a rise in steady state levels of cyclic AMP in the cerebrum and cerebellum subsequent to sacrifice by focused microwave irradiation. One hour pretreatment with anticonvulsant drugs - phenytoin, phenobarbital, carbamazepine, clonazepam, and diazepam - prevented this pentylenetetrazol-induced rise in cyclic AMP. Anticonvulsant agents which alone depressed steady state levels of cyclic AMP were phenytoin and carbamazepine in the cerebral cortex and carbamazepine in the cerebellum while elevations in the cyclic nucleotide were evoked by clonazepam in the cerebrum and cerebellum or diazepam and phenytoin in the cerebellum. Under in vitro conditions, in which incubated tissue slices of mouse cerebral cortex were used, the stimulation of cyclic AMP by norepinephrine was blocked by carbamazepine>phenobarbital>phenytoin. Inhibition of adenosine-induced accumulation of cyclic AMP was observed with phenobarbital>carbamazepine>phenytoin. Clonazepam enhanced this response to adenosine. Ouabain stimulation of cyclic AMP was prevented by carbamazepine, phenytoin>clonazepam>phenobarbital>diazepam. Only the highest concentrations (0.5 mM) of phenytoin, phenobarbital, and carbamazepine reduced the action of KCl on cyclic AMP elevation. Clonazepam and diazepam elevated the basal levels of the cyclic nucleotide.

Original languageEnglish (US)
Pages (from-to)95-104
Number of pages10
JournalEpilepsia
Volume20
Issue number2
StatePublished - 1979

Fingerprint

Carbamazepine
Phenytoin
Cyclic AMP
Anticonvulsants
Clonazepam
Phenobarbital
Diazepam
Brain
Cerebellum
Pentylenetetrazole
Cyclic Nucleotides
Cerebrum
Cerebral Cortex
Adenosine
Ouabain
Microwaves
In Vitro Techniques
Norepinephrine
Injections

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Palmer, G. C., Jones, D. J., Medina, M. A., & Stavinoha, W. B. (1979). Anticonvulsant drug actions on in vitro and in vivo levels of cyclic AMP in the mouse brain. Epilepsia, 20(2), 95-104.

Anticonvulsant drug actions on in vitro and in vivo levels of cyclic AMP in the mouse brain. / Palmer, G. C.; Jones, D. J.; Medina, M. A.; Stavinoha, W. B.

In: Epilepsia, Vol. 20, No. 2, 1979, p. 95-104.

Research output: Contribution to journalArticle

Palmer, GC, Jones, DJ, Medina, MA & Stavinoha, WB 1979, 'Anticonvulsant drug actions on in vitro and in vivo levels of cyclic AMP in the mouse brain', Epilepsia, vol. 20, no. 2, pp. 95-104.
Palmer GC, Jones DJ, Medina MA, Stavinoha WB. Anticonvulsant drug actions on in vitro and in vivo levels of cyclic AMP in the mouse brain. Epilepsia. 1979;20(2):95-104.
Palmer, G. C. ; Jones, D. J. ; Medina, M. A. ; Stavinoha, W. B. / Anticonvulsant drug actions on in vitro and in vivo levels of cyclic AMP in the mouse brain. In: Epilepsia. 1979 ; Vol. 20, No. 2. pp. 95-104.
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