Anticandidal activity and biocompatibility of a rechargeable antifungal denture material

C. C. Villar, A. L. Lin, Z. Cao, X. R. Zhao, L. A. Wu, Shuo Chen, Y. Sun, Chih-ko Yeh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

OBJECTIVES: Candida-associated denture stomatitis is a recurrent and debilitating oral mucosal disease. Development of anticandidal denture materials represents a promising strategy to manage this condition. We have previously shown that miconazole incorporated in methacrylic acid (MAA) copolymerized diurethane dimethacrylate (UDMA) denture materials has longterm anticandidal activity. In this study, we examined the ability of culture medium conditioned with drugfree- or miconazole-MAA-UDMA discs to prevent Candida infection in an in vitro oral epithelial cell/Candida albicans coculture system. MATERIALS AND METHODS: Candida albicans (C. albicans)- induced OKF6/TERT-2 cell damage was quantified by the release of lactate dehydrogenase from epithelial cells, cytokine production was quantified using protein cytokine arrays, and the expression of C. albicans genes was measured by RT-qPCR. RESULTS: Candida albicans had limited growth with altered expression levels of secreted aspartyl proteinase-2 and -5 in culture medium conditioned by miconazole- MAA-UDMA discs. Significantly, the ability of C. albicans to induce oral epithelial cell damage and trigger epithelial proinflammatory cytokine production was also inhibited by miconazole disc conditioned media. CONCLUSION: Miconazole released from MAA-UDMA denture materials effectively prevents the development of candidal infection in an in vitro oral epithelial system. Further characterization of this drug-rechargeable denture material is warranted.

Original languageEnglish (US)
Pages (from-to)287-295
Number of pages9
JournalOral Diseases
Volume19
Issue number3
DOIs
StatePublished - 2013

Fingerprint

Miconazole
Dentures
Candida albicans
Conditioned Culture Medium
Epithelial Cells
Cytokines
Candida
Mouth Diseases
Denture Stomatitis
Aspartic Acid Proteases
Protein Array Analysis
Coculture Techniques
Infection
L-Lactate Dehydrogenase
methacrylic acid
Growth
Pharmaceutical Preparations
Genes

Keywords

  • Anticandidal
  • Candida albicans
  • Denture stomatitis
  • Epithelial cells
  • Rechargeable denture material

ASJC Scopus subject areas

  • Dentistry(all)
  • Otorhinolaryngology

Cite this

Anticandidal activity and biocompatibility of a rechargeable antifungal denture material. / Villar, C. C.; Lin, A. L.; Cao, Z.; Zhao, X. R.; Wu, L. A.; Chen, Shuo; Sun, Y.; Yeh, Chih-ko.

In: Oral Diseases, Vol. 19, No. 3, 2013, p. 287-295.

Research output: Contribution to journalArticle

Villar, C. C. ; Lin, A. L. ; Cao, Z. ; Zhao, X. R. ; Wu, L. A. ; Chen, Shuo ; Sun, Y. ; Yeh, Chih-ko. / Anticandidal activity and biocompatibility of a rechargeable antifungal denture material. In: Oral Diseases. 2013 ; Vol. 19, No. 3. pp. 287-295.
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