Abstract
Hemagglutination is of questionable validity for following the synthesis of 19S to 7S chicken anti-BSA antibodies, since only small amounts of the major antibody population (7S) are measured in early primary antisera. The 7S antibodies in primary antisera were demonstrated by antigen-binding methods (ammonium sulfate salting out procedure and RIE). Radioimmunoelec-trophoretic patterns localized anti-BSA and anti-HSA primary antibody activities in the 7S γ−, γ1A−, and γ1M−globulins; however, only a few of these sera had γ1A− activity. No γ1M− antibody was detected by RIE in hyperim-mune antisera, All anti-azobenzoate antisera had γ− and γ1M−globulin antibodies, but no activity in the γ1M−globulin. The species of chicken antibody synthesized seems to be related to the nature of the immunizing antigen.
Original language | English (US) |
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Pages (from-to) | 292-296 |
Number of pages | 5 |
Journal | Proceedings of the Society for Experimental Biology and Medicine |
Volume | 118 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1965 |
Externally published | Yes |
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology