Antibody Activity in Different Chicken Globulins

Gordon Dreesman, Charles Larson, R. Neal Pinckard, Rose Mary Groyon, Albert A. Benedict

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Hemagglutination is of questionable validity for following the synthesis of 19S to 7S chicken anti-BSA antibodies, since only small amounts of the major antibody population (7S) are measured in early primary antisera. The 7S antibodies in primary antisera were demonstrated by antigen-binding methods (ammonium sulfate salting out procedure and RIE). Radioimmunoelec-trophoretic patterns localized anti-BSA and anti-HSA primary antibody activities in the 7S γ−, γ1A−, and γ1M−globulins; however, only a few of these sera had γ1A− activity. No γ1M− antibody was detected by RIE in hyperim-mune antisera, All anti-azobenzoate antisera had γ− and γ1M−globulin antibodies, but no activity in the γ1M−globulin. The species of chicken antibody synthesized seems to be related to the nature of the immunizing antigen.

Original languageEnglish (US)
Pages (from-to)292-296
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume118
Issue number1
DOIs
StatePublished - Jan 1965
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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