TY - JOUR
T1 - Antibody Activity in Different Chicken Globulins
AU - Dreesman, Gordon
AU - Larson, Charles
AU - Pinckard, R. Neal
AU - Groyon, Rose Mary
AU - Benedict, Albert A.
N1 - Funding Information:
Supported in part by USPHS Training Grant 1 T1 AI 243-01 and USPHS Fellowship l-FI-GM-21, 099-01 to Gordon Dreesman. Publication No. 39 from Pacific Biomedical Research Center, Univ, of Hawaii.
PY - 1965/1
Y1 - 1965/1
N2 - Hemagglutination is of questionable validity for following the synthesis of 19S to 7S chicken anti-BSA antibodies, since only small amounts of the major antibody population (7S) are measured in early primary antisera. The 7S antibodies in primary antisera were demonstrated by antigen-binding methods (ammonium sulfate salting out procedure and RIE). Radioimmunoelec-trophoretic patterns localized anti-BSA and anti-HSA primary antibody activities in the 7S γ−, γ1A−, and γ1M−globulins; however, only a few of these sera had γ1A− activity. No γ1M− antibody was detected by RIE in hyperim-mune antisera, All anti-azobenzoate antisera had γ− and γ1M−globulin antibodies, but no activity in the γ1M−globulin. The species of chicken antibody synthesized seems to be related to the nature of the immunizing antigen.
AB - Hemagglutination is of questionable validity for following the synthesis of 19S to 7S chicken anti-BSA antibodies, since only small amounts of the major antibody population (7S) are measured in early primary antisera. The 7S antibodies in primary antisera were demonstrated by antigen-binding methods (ammonium sulfate salting out procedure and RIE). Radioimmunoelec-trophoretic patterns localized anti-BSA and anti-HSA primary antibody activities in the 7S γ−, γ1A−, and γ1M−globulins; however, only a few of these sera had γ1A− activity. No γ1M− antibody was detected by RIE in hyperim-mune antisera, All anti-azobenzoate antisera had γ− and γ1M−globulin antibodies, but no activity in the γ1M−globulin. The species of chicken antibody synthesized seems to be related to the nature of the immunizing antigen.
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U2 - 10.3181/00379727-118-29823
DO - 10.3181/00379727-118-29823
M3 - Article
C2 - 14254573
AN - SCOPUS:78651169967
VL - 118
SP - 292
EP - 296
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 1535-3702
IS - 1
ER -