Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes

Alfred Nordheim, Mary Lou Pardue, Eileen M. Lafer, Achim Möller, B. David Stollar, Alexander Rich

Research output: Contribution to journalArticlepeer-review

235 Scopus citations

Abstract

Antibodies which are specific to the Z-DNA conformation have been purified and characterized on the basis of their binding to three different DNA polymers which can form this left-handed helix. These antibodies bind specifically to polytene chromosomes of Drosophila melanogaster as visualized by fluorescent staining. The staining is found in the interband regions and its intensity varies among different interbands in a reproducible manner. This is the first identification of the Z-DNA conformation in material of biological origin.

Original languageEnglish (US)
Pages (from-to)417-422
Number of pages6
JournalNature
Volume294
Issue number5840
DOIs
StatePublished - 1981
Externally publishedYes

ASJC Scopus subject areas

  • General

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