Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes

Alfred Nordheim; Mary Lou Pardue; Eileen M. Lafer; Achim Möller; B. David Stollar; Alexander Rich

doi:10.1038/294417a0

Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes

Alfred Nordheim, Mary Lou Pardue, Eileen M. Lafer, Achim Möller, B. David Stollar, Alexander Rich

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Abstract

Antibodies which are specific to the Z-DNA conformation have been purified and characterized on the basis of their binding to three different DNA polymers which can form this left-handed helix. These antibodies bind specifically to polytene chromosomes of Drosophila melanogaster as visualized by fluorescent staining. The staining is found in the interband regions and its intensity varies among different interbands in a reproducible manner. This is the first identification of the Z-DNA conformation in material of biological origin.

Original language	English (US)
Pages (from-to)	417-422
Number of pages	6
Journal	Nature
Volume	294
Issue number	5840
DOIs	https://doi.org/10.1038/294417a0
State	Published - 1981
Externally published	Yes

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abstract = "Antibodies which are specific to the Z-DNA conformation have been purified and characterized on the basis of their binding to three different DNA polymers which can form this left-handed helix. These antibodies bind specifically to polytene chromosomes of Drosophila melanogaster as visualized by fluorescent staining. The staining is found in the interband regions and its intensity varies among different interbands in a reproducible manner. This is the first identification of the Z-DNA conformation in material of biological origin.",

author = "Alfred Nordheim and Pardue, {Mary Lou} and Lafer, {Eileen M.} and Achim M{\"o}ller and Stollar, {B. David} and Alexander Rich",

year = "1981",

doi = "10.1038/294417a0",

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pages = "417--422",

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T1 - Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes

AU - Nordheim, Alfred

AU - Pardue, Mary Lou

AU - Lafer, Eileen M.

AU - Möller, Achim

AU - Stollar, B. David

AU - Rich, Alexander

PY - 1981

Y1 - 1981

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AB - Antibodies which are specific to the Z-DNA conformation have been purified and characterized on the basis of their binding to three different DNA polymers which can form this left-handed helix. These antibodies bind specifically to polytene chromosomes of Drosophila melanogaster as visualized by fluorescent staining. The staining is found in the interband regions and its intensity varies among different interbands in a reproducible manner. This is the first identification of the Z-DNA conformation in material of biological origin.

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ER -

Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes

Abstract

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