Antibodies against Z DNA react with the macronucleus but not the micronucleus of the hypotrichous ciliate stylonychia mytilus

Hans J. Lipps, Alfred Nordheim, Eileen M Lafer, Dieter Ammermann, B. David Stollar, Alexander Rich

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Using indirect immunofluorescence, we studied the reaction of antibodies specific for left-handed Z DNA with the nuclei of the hypotrichous ciliate Stylonychia mytilus. In the vegetative cell, the macronucleus reacts strongly with these antibodies, but no reaction can be detected with micronuclei. However, an antibody that binds to denatured and right-handed B DNA reacts with both types of nuclei. No reaction of the anti-Z DNA antibody is seen in the macronuclear replication band. Digestion of macronuclei with DNAase I leads to a decrease in the anti-Z DNA antibody reaction. Some stages of the developing macronucleus were also investigated. No reaction is seen at the polytene chromosome stage, but following DNA elimination the nucleus is seen to react with the antibody.

Original languageEnglish (US)
Pages (from-to)435-441
Number of pages7
JournalCell
Volume32
Issue number2
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Macronucleus
Z-Form DNA
Mytilus
Antibodies
Antinuclear Antibodies
Polytene Chromosomes
Deoxyribonuclease I
DNA
Indirect Fluorescent Antibody Technique
Digestion
Chromosomes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Antibodies against Z DNA react with the macronucleus but not the micronucleus of the hypotrichous ciliate stylonychia mytilus. / Lipps, Hans J.; Nordheim, Alfred; Lafer, Eileen M; Ammermann, Dieter; Stollar, B. David; Rich, Alexander.

In: Cell, Vol. 32, No. 2, 1983, p. 435-441.

Research output: Contribution to journalArticle

Lipps, Hans J. ; Nordheim, Alfred ; Lafer, Eileen M ; Ammermann, Dieter ; Stollar, B. David ; Rich, Alexander. / Antibodies against Z DNA react with the macronucleus but not the micronucleus of the hypotrichous ciliate stylonychia mytilus. In: Cell. 1983 ; Vol. 32, No. 2. pp. 435-441.
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AU - Ammermann, Dieter

AU - Stollar, B. David

AU - Rich, Alexander

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