TY - JOUR
T1 - Antibiofilm activity of an EDTA-containing nanoemulsion on multidrug-resistant Acinetobacter baumannii
AU - Ramalingam, Karthikeyan
AU - Lee, Valerie A.
N1 - Funding Information:
Sincere appreciation is extended to the authorities of the Naval Medical Research Unit San Antonio (NAMRU-SA), San Antonio, TX and DST Science and Engineering Research Board (SERB) [Grant number SERB/LS-267/2014] and Extra Mural Research Funding of AYUSH (Ministry of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy [Grant number Z. 28015/209/2015-HPC] for providing funds and facilities to perform this work. We also want to acknowledge Prof. H. Ralph Rawls, Department of Comprehensive Dentistry, University of Texas Health at San Antonio, for his valuable guidance.
Funding Information:
Sincere appreciation is extended to the authorities of the Naval Medical Research Unit San Antonio (NAMRU-SA), San Antonio, TX and DST Science and Engineering Research Board (SERB) [Grant number SERB/LS-267/2014] and Extra Mural Research Funding of AYUSH (Ministry of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy [Grant number Z. 28015/209/2015-HPC] for providing funds and facilities to perform this work.
Publisher Copyright:
© 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2018/11/5
Y1 - 2018/11/5
N2 - Acinetobacter baumannii have evolved as an exceedingly troublesome pathogenic microorganisms and prevention and controlling this pathogen is considered to be a public health problem. Nanoemulsions (NE) are a distinctive type of decontaminator produced by integration of immiscible oil phase with aqueous phase under extreme shear forces. The effectiveness of NEs and their components was determined against four stains of A. baumannii by MBC, adherence assay, biofilm assay and SEM studies. NE dilutions ranging from 125 to 225 reduced adhesion by from 61.8 to 99.9% in NE-treated groups (p<.05) as determined by MBC. Four-day-old A. baumannii biofilms were treated with NE; LIVE/DEAD staining showed dead cell intensity of 56.2–92.0% in NE-treated groups. After NE treatment and observation by SEM, cell surfaces appeared to be remarkably disintegrated. Irregular boundaries were observed and margins of cell walls were unclear. The anti-adherence, anti-biofilm and morphological disruption effects of NE suggest that this material could be useful for the development of promising antimicrobial agents.
AB - Acinetobacter baumannii have evolved as an exceedingly troublesome pathogenic microorganisms and prevention and controlling this pathogen is considered to be a public health problem. Nanoemulsions (NE) are a distinctive type of decontaminator produced by integration of immiscible oil phase with aqueous phase under extreme shear forces. The effectiveness of NEs and their components was determined against four stains of A. baumannii by MBC, adherence assay, biofilm assay and SEM studies. NE dilutions ranging from 125 to 225 reduced adhesion by from 61.8 to 99.9% in NE-treated groups (p<.05) as determined by MBC. Four-day-old A. baumannii biofilms were treated with NE; LIVE/DEAD staining showed dead cell intensity of 56.2–92.0% in NE-treated groups. After NE treatment and observation by SEM, cell surfaces appeared to be remarkably disintegrated. Irregular boundaries were observed and margins of cell walls were unclear. The anti-adherence, anti-biofilm and morphological disruption effects of NE suggest that this material could be useful for the development of promising antimicrobial agents.
KW - Acinetobacter baumannii
KW - Biofilm
KW - EDTA
KW - antimicrobial agents
KW - nanoemulsion
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U2 - 10.1080/21691401.2018.1468771
DO - 10.1080/21691401.2018.1468771
M3 - Article
AN - SCOPUS:85046440864
VL - 46
SP - 737
EP - 743
JO - Artificial Cells, Nanomedicine and Biotechnology
JF - Artificial Cells, Nanomedicine and Biotechnology
SN - 2169-1401
IS - sup2
ER -