Anti-peptide antibodies detect a lupus-related interspecies idiotype THAT maps to H chain CDR2

Katheryn Meek, Masami Takei, Howard Dang, Inaki Sanz, Michael J. Dauphinee, J. Donald Capra, Norman Talal

Research output: Contribution to journalArticle

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Abstract

Antibodies to the small nucleoprotein Sm occur spontaneously in human and murine systemic lupus erythematosus. Human and mouse monoclonal anti-Sm autoantibodies designated 4B4 and Y2 share an idiotype (Id) determinant located on the Ig H chain. To understand the molecular basis of this cross-reactivity, the VH regions of both antibodies were sequenced and analyzed for homology. The antibodies showed only 49.6% homology. The second complementary determining region (CDR2) was the most likely candidate for the Id site. To investigate this possibility, rabbit antiserum was made against a peptide corresponding to the CDR2 of 4B4. This antiserum was specific for the immunizing peptide and reacted weakly to a peptide corresponding to the CDR2 of Y2. Anti-CDR2 antibody bound to 4B4 and Y2 but not to other human and mouse mAb. Binding was directed at the H chain when analyzed by Western blots. Anti-CDR2 antibody blocked anti-Id antibody binding to 4B4 and Y2 by 58% and 24%, respectively. These studies suggest that this interspecies Id maps to the H chain CDR2 and that a conserved Id can occur within molecules that are otherwise radically different.

Original languageEnglish (US)
Pages (from-to)1375-1381
Number of pages7
JournalJournal of Immunology
Volume144
Issue number4
StatePublished - Feb 15 1990

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Anti-Idiotypic Antibodies
Peptides
Antibodies
Immune Sera
Nucleoproteins
Systemic Lupus Erythematosus
Autoantibodies
Western Blotting
Rabbits

ASJC Scopus subject areas

  • Immunology

Cite this

Meek, K., Takei, M., Dang, H., Sanz, I., Dauphinee, M. J., Capra, J. D., & Talal, N. (1990). Anti-peptide antibodies detect a lupus-related interspecies idiotype THAT maps to H chain CDR2. Journal of Immunology, 144(4), 1375-1381.

Anti-peptide antibodies detect a lupus-related interspecies idiotype THAT maps to H chain CDR2. / Meek, Katheryn; Takei, Masami; Dang, Howard; Sanz, Inaki; Dauphinee, Michael J.; Capra, J. Donald; Talal, Norman.

In: Journal of Immunology, Vol. 144, No. 4, 15.02.1990, p. 1375-1381.

Research output: Contribution to journalArticle

Meek, K, Takei, M, Dang, H, Sanz, I, Dauphinee, MJ, Capra, JD & Talal, N 1990, 'Anti-peptide antibodies detect a lupus-related interspecies idiotype THAT maps to H chain CDR2', Journal of Immunology, vol. 144, no. 4, pp. 1375-1381.
Meek K, Takei M, Dang H, Sanz I, Dauphinee MJ, Capra JD et al. Anti-peptide antibodies detect a lupus-related interspecies idiotype THAT maps to H chain CDR2. Journal of Immunology. 1990 Feb 15;144(4):1375-1381.
Meek, Katheryn ; Takei, Masami ; Dang, Howard ; Sanz, Inaki ; Dauphinee, Michael J. ; Capra, J. Donald ; Talal, Norman. / Anti-peptide antibodies detect a lupus-related interspecies idiotype THAT maps to H chain CDR2. In: Journal of Immunology. 1990 ; Vol. 144, No. 4. pp. 1375-1381.
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