Anti-peptide antibodies detect a lupus-related interspecies idiotype that maps to H chain CDR2

K. Meek, M. Takei, H. Dang, I. Sanz, M. J. Dauphinee, J. D. Capra, N. Talal

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Antibodies to the small nucleoprotein Sm occur spontaneously in human and murine systemic lupus erythematosus. Human and mouse monoclonal anti-Sm autoantibodies designated 4B4 and Y2 share an idiotype (Id) determinant located on the Ig H chain. To understand the molecular basis of this cross-reactivity, the VH regions of both antibodies were sequenced and analyzed for homology. The antibodies showed only 49.6% homology. The second complementary determining region (CDR2) was the most likely candidate for the Id site. To investigate this possibility, rabbit antiserum was made against a peptide corresponding to the CDR2 of 4B4. This antiserum was specific for the immunizing peptide and reacted weakly to a peptide corresponding to the CDR2 of Y2. Anti-CDR2 antibody bound to 4B4 and Y2 but not to other human and mouse mAb. Binding was directed at the H chain when analyzed by Western blots. Anti-CDR2 antibody blocked anti-Id antibody binding to 4B4 and Y2 by 58% and 24%, respectively. These studies suggest that this interspecies Id maps to the H chain CDR2 and that a conserved Id can occur within molecules that are otherwise radically different.

Original languageEnglish (US)
Pages (from-to)1375-1381
Number of pages7
JournalJournal of Immunology
Volume144
Issue number4
StatePublished - 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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