Anti-inflammatory action of dietary fish oil and calorie restriction

You Jung Kim, Hyon Jeen Kim, Jae Kyung No, Hae Young Chung, Gabriel Fernandes

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Inflammation, inflammatory mediators, cyclooxygenase (COX)-2, and inducible nitric oxide (iNOS) are all influenced by age-related oxidative status. To investigate the effect of dietary fish oil (FO) and calorie restriction (CR) on oxidative stress-related inflammatory status with age, (NZB/NZW) F1 (B/W) mice were fed for 4 and 9 months either ad libitum or calorie-restricted (60% of ad libitum intake) diets containing 5% corn oil or 5% FO. We measured several key oxidative and inflammatory markers: TBARS, xanthine oxidase (XOD)-derived superoxide generation, and PGE2 and LTB4 production. Expressions of renal COX-1, COX-2, and iNOS mRNA were analyzed by RT-PCR; additionally, COX-2 protein was estimated by Western-blot method. Results show that FO intake and CR individually and together suppressed age-related increases in lipid peroxidation and superoxide generation. The inhibitory effects of dietary FO and CR were also found for iNOS expression, COX-2 expression, which subsequently led to the suppression of PGE2 and LTB4. We conclude that the beneficial effects of FO feeding and CR are synergistic in ameliorating the age-related nephritis of B/W mice by suppressing COX-2 and iNOS, reactive species generation, and pro-inflammatory mediators.

Original languageEnglish (US)
Pages (from-to)2523-2532
Number of pages10
JournalLife Sciences
Volume78
Issue number21
DOIs
StatePublished - Apr 18 2006

Keywords

  • Calorie restriction
  • Cyclooxygenase
  • Dietary fish oil
  • Inducible nitric oxide synthase
  • Inflammatory mediator
  • Reactive species

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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