Anomalies in polysome profiles caused by contamination of the gradients with Cu2+ or Zn2+

Evelyn McGown, Arlan Richardson, L. M. Henderson, Patricia B. Swan

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The presence of 30 μM Cu2+ or Zn2+ in sucrose gradients used to determine the extent of ribosome aggregation in rat liver preparations resulted in profiles which were abnormally aggregated. This problem was eliminated by inclusion of EDTA in the gradient or by the use of detergents other than deoxycholate.

Original languageEnglish (US)
Pages (from-to)165-169
Number of pages5
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume247
Issue number1
DOIs
StatePublished - Sep 30 1971

ASJC Scopus subject areas

  • Medicine(all)

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