Annexin 2: A novel human immunodeficiency virus type 1 gag binding protein involved in replication in monocyte-derived macrophages

Elena V. Ryzhova, Robin M. Vos, Andrew V. Albright, Alexia V. Harrist, Thomas Harvey, Francisco González-Scarano

Research output: Contribution to journalArticle

80 Scopus citations


Human immunodeficiency virus (HIV) replication in the major natural target cells, CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life cycle. However, it differs as to viral assembly and budding, which take place on plasma membranes in T cells and on endosomal membranes in macrophages. It has been postulated that cell type-specific host factors may aid in directing viral assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to productively infected macrophages and was not detected in quiescently infected monocyte-derived macrophages (MDM) in which an HIV replication block was mapped to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F. Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag interaction likely occurs at the limiting membranes of late endosomes/multivesicular bodies and that Anx2 depletion is associated with a significant decline in the infectivity of released virions; this coincided with incomplete Gag processing and inefficient incorporation of CD63. Cumulatively, our data suggest that Anx2 is essential for the proper assembly of HIV in MDM.

Original languageEnglish (US)
Pages (from-to)2694-2704
Number of pages11
JournalJournal of Virology
Issue number6
Publication statusPublished - Mar 1 2006


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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