Anguidine potentiates cis-platinum in human brain tumor cells

Robert A. Hromas, WK Alfred Yung

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Anguidine pretreatment was previously shown to potentiate cis-platinum in Chinese hamster ovary cells by 100-fold, probably by enhancing cellular cis-platinum. uptake. Since both cis-platinum and anguidine have been reported to have clinical efficacy in human brain tumors, the present study was initiated to investigate whether anguidine's potentiation of cis-platinum was applicable to human brain tumor cells in culture. Using the colony formation assay, it was found that anguidine enhanced cis-platinum's cytotoxicity by ten-fold, producing a dose modification factor of 1.74. Alkaline elution analysis of cis-platinum-induced DNA crosslinks found that anguidine enhanced cross-linking by a factor of 1.55, 1.76, 1.63, and 1.48 at 0, 6, 24, and 48 hr, respectively, after cis-platinum treatment. This enhancement of cross-linking is evidence for anguidine increasing cis-platinum uptake. Thus, anguidine enhances cis-platinum-induced DNA cross-linking and subsequent cytotoxicity in human brain tumor cells, and may be clinically useful in combination with cis-platinum in those tumors.

Original languageEnglish (US)
Pages (from-to)343-348
Number of pages6
JournalJournal of Neuro-Oncology
Volume3
Issue number4
DOIs
StatePublished - Dec 1 1986
Externally publishedYes

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Keywords

  • DNA cross-linking
  • anguidine
  • brain tumors
  • cis-platinum

ASJC Scopus subject areas

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

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