Angiotensin II inhibits insulin-stimulated phosphorylation of eukaryotic initiation factor 4E-binding protein-1 in proximal tubular epithelial cells

D. Senthil, J. L. Faulkner, Goutam Ghosh-choudhury, H. E. Abboud, Balakuntalam S Kasinath

Research output: Contribution to journalArticle

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Abstract

Interaction between angiotensin II, which binds a G-protein-coupled receptor, and insulin, a ligand for receptor tyrosine kinase, was examined in renal proximal tubular epithelial cells. Augmented protein translation by insulin involves activation of eukaryotic initiation factor 4E (eIF4E) which follows the release of the factor from a heterodimeric complex by phosphorylation of its binding protein, 4E-BP1. Angiotensin II (1 nM) or insulin (1 nM) individually stimulated 4E-BP1 phosphorylation. However, pre-incubation with angiotensin II abrogated insulin-induced phosphorylation of 4E-BP1, resulting in persistent binding to eIF4E. Although angiotensin II and insulin individually activated phosphoinositide 3-kinase and extracellular signal-regulated kinase (ERK)-1/-2-type mitogen-activated protein (MAP) kinase, pre-incubation with angiotensin II abolished insulin-induced stimulation of these kinases, suggesting more proximal events in insulin signalling may be intercepted. Pre-treatment with angiotensin II markedly inhibited insulin-stimulated tyrosine phosphorylation of insulin-receptor β-chain and insulin-receptor substrate 1. Losartan prevented angiotensin II inhibition of insulin-induced ERK-1/-2-type MAP kinase activation and 4E-BP1 phosphorylation, suggesting mediation of the effect of angiotensin II by its type 1 receptor. Insulin-stimulated de novo protein synthesis was also abolished by pre-incubation with angiotensin II. These data show that angiotensin II inhibits 4E-BP1 phosphorylation and stimulation of protein synthesis induced by insulin by interfering with proximal events in insulin signalling. Our data provide a mechanistic basis for insulin insensitivity induced by angiotensin II.

Original languageEnglish (US)
Pages (from-to)87-95
Number of pages9
JournalBiochemical Journal
Volume360
Issue number1
DOIs
StatePublished - Nov 15 2001

Fingerprint

Eukaryotic Initiation Factor-4E
Phosphorylation
Angiotensin II
Carrier Proteins
Epithelial Cells
Insulin
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinases
Chemical activation
Insulin Receptor Substrate Proteins
Angiotensin Type 1 Receptor
Proteins
1-Phosphatidylinositol 4-Kinase
Losartan
Insulin Receptor
Receptor Protein-Tyrosine Kinases
Protein Biosynthesis
G-Protein-Coupled Receptors
Phosphatidylinositols

Keywords

  • 4E-BP1 phosphorylation
  • Receptor cross-talk

ASJC Scopus subject areas

  • Biochemistry

Cite this

Angiotensin II inhibits insulin-stimulated phosphorylation of eukaryotic initiation factor 4E-binding protein-1 in proximal tubular epithelial cells. / Senthil, D.; Faulkner, J. L.; Ghosh-choudhury, Goutam; Abboud, H. E.; Kasinath, Balakuntalam S.

In: Biochemical Journal, Vol. 360, No. 1, 15.11.2001, p. 87-95.

Research output: Contribution to journalArticle

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