Angiogenic bFGF expression from gas-plasma treated scaffolds

Steven R Bailey, Jodie L. Polan, Brian Morse, Suzanne Wetherhold, Rosa E. Villanueva-Vedia, Douglas Waggoner, Clyde Phelix, Edwin Barera-Roderiquiz, Nilesh Goswami, Oscar Munoz, C. Mauli Agrawal

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: In vivo experiments indicate that gas-plasma-treated D,L-polylactide polymers expressing basic fibroblast growth factor (bFGF) exhibit enhanced angiogenesis. bFGF is not a single entity, but it is instead a family of isoforms. Consequently, we sought to determine which bFGF isoforms and levels initiate angiogenesis in nude mice peritoneums. Methods: Cytoplasmic and nuclear bFGF were characterized for nude mice peritoneums incubated with nontreated scaffolds containing HAEC (CW), its respective polymer-only scaffolds (Cp) and gas-plasma treated scaffolds with HAEC (TW) and without cells (Tp). NuPAGE electrophoresis and WesternBreeze Chemiluminescent kits were used to analyze relative bFGF densities and molecular weights. VEGF was quantified using ImageJ. Results: bFGF bands were located at molecular weights of 24, 48, 58, 72 and 80 kDa, depending on whether they were from cytoplasms or nuclei. At 12, 24 and 72 days, 58-kDa bFGF bands were observed from nuclei of TW and Tp, 80-kDa bFGF bands were only observed in cytoplasmic fractions ≤24 days. Total cytoplasmic and nuclear bFGF intensities increased from 12 to 24 days, then declined by 72 days. Conclusions: (1) Gas-plasma treated scaffolds up-regulate bFGF isoforms. (2) bFGF was expressed in the nuclei; however, 80-kDa bFGF was seen only in cytoplasms.

Original languageEnglish (US)
Pages (from-to)183-189
Number of pages7
JournalCardiovascular Radiation Medicine
Volume3
Issue number3-4
DOIs
StatePublished - Jul 2002

Fingerprint

Plasma Gases
Fibroblast Growth Factor 2
Protein Isoforms
Nude Mice
Polymers
Cytoplasm
Molecular Weight
Vascular Endothelial Growth Factor A
Electrophoresis

Keywords

  • Angiogenesis
  • Basic fibroblast growth factor
  • Bioresorbable scaffolds
  • Gas-plasma treatment

ASJC Scopus subject areas

  • Molecular Medicine
  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Bailey, S. R., Polan, J. L., Morse, B., Wetherhold, S., Villanueva-Vedia, R. E., Waggoner, D., ... Agrawal, C. M. (2002). Angiogenic bFGF expression from gas-plasma treated scaffolds. Cardiovascular Radiation Medicine, 3(3-4), 183-189. https://doi.org/10.1016/S1522-1865(03)00098-2

Angiogenic bFGF expression from gas-plasma treated scaffolds. / Bailey, Steven R; Polan, Jodie L.; Morse, Brian; Wetherhold, Suzanne; Villanueva-Vedia, Rosa E.; Waggoner, Douglas; Phelix, Clyde; Barera-Roderiquiz, Edwin; Goswami, Nilesh; Munoz, Oscar; Agrawal, C. Mauli.

In: Cardiovascular Radiation Medicine, Vol. 3, No. 3-4, 07.2002, p. 183-189.

Research output: Contribution to journalArticle

Bailey, SR, Polan, JL, Morse, B, Wetherhold, S, Villanueva-Vedia, RE, Waggoner, D, Phelix, C, Barera-Roderiquiz, E, Goswami, N, Munoz, O & Agrawal, CM 2002, 'Angiogenic bFGF expression from gas-plasma treated scaffolds', Cardiovascular Radiation Medicine, vol. 3, no. 3-4, pp. 183-189. https://doi.org/10.1016/S1522-1865(03)00098-2
Bailey SR, Polan JL, Morse B, Wetherhold S, Villanueva-Vedia RE, Waggoner D et al. Angiogenic bFGF expression from gas-plasma treated scaffolds. Cardiovascular Radiation Medicine. 2002 Jul;3(3-4):183-189. https://doi.org/10.1016/S1522-1865(03)00098-2
Bailey, Steven R ; Polan, Jodie L. ; Morse, Brian ; Wetherhold, Suzanne ; Villanueva-Vedia, Rosa E. ; Waggoner, Douglas ; Phelix, Clyde ; Barera-Roderiquiz, Edwin ; Goswami, Nilesh ; Munoz, Oscar ; Agrawal, C. Mauli. / Angiogenic bFGF expression from gas-plasma treated scaffolds. In: Cardiovascular Radiation Medicine. 2002 ; Vol. 3, No. 3-4. pp. 183-189.
@article{43a26317894c4da9a41df7c0e8c8e83e,
title = "Angiogenic bFGF expression from gas-plasma treated scaffolds",
abstract = "Purpose: In vivo experiments indicate that gas-plasma-treated D,L-polylactide polymers expressing basic fibroblast growth factor (bFGF) exhibit enhanced angiogenesis. bFGF is not a single entity, but it is instead a family of isoforms. Consequently, we sought to determine which bFGF isoforms and levels initiate angiogenesis in nude mice peritoneums. Methods: Cytoplasmic and nuclear bFGF were characterized for nude mice peritoneums incubated with nontreated scaffolds containing HAEC (CW), its respective polymer-only scaffolds (Cp) and gas-plasma treated scaffolds with HAEC (TW) and without cells (Tp). NuPAGE electrophoresis and WesternBreeze Chemiluminescent kits were used to analyze relative bFGF densities and molecular weights. VEGF was quantified using ImageJ. Results: bFGF bands were located at molecular weights of 24, 48, 58, 72 and 80 kDa, depending on whether they were from cytoplasms or nuclei. At 12, 24 and 72 days, 58-kDa bFGF bands were observed from nuclei of TW and Tp, 80-kDa bFGF bands were only observed in cytoplasmic fractions ≤24 days. Total cytoplasmic and nuclear bFGF intensities increased from 12 to 24 days, then declined by 72 days. Conclusions: (1) Gas-plasma treated scaffolds up-regulate bFGF isoforms. (2) bFGF was expressed in the nuclei; however, 80-kDa bFGF was seen only in cytoplasms.",
keywords = "Angiogenesis, Basic fibroblast growth factor, Bioresorbable scaffolds, Gas-plasma treatment",
author = "Bailey, {Steven R} and Polan, {Jodie L.} and Brian Morse and Suzanne Wetherhold and Villanueva-Vedia, {Rosa E.} and Douglas Waggoner and Clyde Phelix and Edwin Barera-Roderiquiz and Nilesh Goswami and Oscar Munoz and Agrawal, {C. Mauli}",
year = "2002",
month = "7",
doi = "10.1016/S1522-1865(03)00098-2",
language = "English (US)",
volume = "3",
pages = "183--189",
journal = "Cardiovascular Revascularization Medicine",
issn = "1553-8389",
publisher = "Elsevier Inc.",
number = "3-4",

}

TY - JOUR

T1 - Angiogenic bFGF expression from gas-plasma treated scaffolds

AU - Bailey, Steven R

AU - Polan, Jodie L.

AU - Morse, Brian

AU - Wetherhold, Suzanne

AU - Villanueva-Vedia, Rosa E.

AU - Waggoner, Douglas

AU - Phelix, Clyde

AU - Barera-Roderiquiz, Edwin

AU - Goswami, Nilesh

AU - Munoz, Oscar

AU - Agrawal, C. Mauli

PY - 2002/7

Y1 - 2002/7

N2 - Purpose: In vivo experiments indicate that gas-plasma-treated D,L-polylactide polymers expressing basic fibroblast growth factor (bFGF) exhibit enhanced angiogenesis. bFGF is not a single entity, but it is instead a family of isoforms. Consequently, we sought to determine which bFGF isoforms and levels initiate angiogenesis in nude mice peritoneums. Methods: Cytoplasmic and nuclear bFGF were characterized for nude mice peritoneums incubated with nontreated scaffolds containing HAEC (CW), its respective polymer-only scaffolds (Cp) and gas-plasma treated scaffolds with HAEC (TW) and without cells (Tp). NuPAGE electrophoresis and WesternBreeze Chemiluminescent kits were used to analyze relative bFGF densities and molecular weights. VEGF was quantified using ImageJ. Results: bFGF bands were located at molecular weights of 24, 48, 58, 72 and 80 kDa, depending on whether they were from cytoplasms or nuclei. At 12, 24 and 72 days, 58-kDa bFGF bands were observed from nuclei of TW and Tp, 80-kDa bFGF bands were only observed in cytoplasmic fractions ≤24 days. Total cytoplasmic and nuclear bFGF intensities increased from 12 to 24 days, then declined by 72 days. Conclusions: (1) Gas-plasma treated scaffolds up-regulate bFGF isoforms. (2) bFGF was expressed in the nuclei; however, 80-kDa bFGF was seen only in cytoplasms.

AB - Purpose: In vivo experiments indicate that gas-plasma-treated D,L-polylactide polymers expressing basic fibroblast growth factor (bFGF) exhibit enhanced angiogenesis. bFGF is not a single entity, but it is instead a family of isoforms. Consequently, we sought to determine which bFGF isoforms and levels initiate angiogenesis in nude mice peritoneums. Methods: Cytoplasmic and nuclear bFGF were characterized for nude mice peritoneums incubated with nontreated scaffolds containing HAEC (CW), its respective polymer-only scaffolds (Cp) and gas-plasma treated scaffolds with HAEC (TW) and without cells (Tp). NuPAGE electrophoresis and WesternBreeze Chemiluminescent kits were used to analyze relative bFGF densities and molecular weights. VEGF was quantified using ImageJ. Results: bFGF bands were located at molecular weights of 24, 48, 58, 72 and 80 kDa, depending on whether they were from cytoplasms or nuclei. At 12, 24 and 72 days, 58-kDa bFGF bands were observed from nuclei of TW and Tp, 80-kDa bFGF bands were only observed in cytoplasmic fractions ≤24 days. Total cytoplasmic and nuclear bFGF intensities increased from 12 to 24 days, then declined by 72 days. Conclusions: (1) Gas-plasma treated scaffolds up-regulate bFGF isoforms. (2) bFGF was expressed in the nuclei; however, 80-kDa bFGF was seen only in cytoplasms.

KW - Angiogenesis

KW - Basic fibroblast growth factor

KW - Bioresorbable scaffolds

KW - Gas-plasma treatment

UR - http://www.scopus.com/inward/record.url?scp=0142227747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142227747&partnerID=8YFLogxK

U2 - 10.1016/S1522-1865(03)00098-2

DO - 10.1016/S1522-1865(03)00098-2

M3 - Article

C2 - 12974371

AN - SCOPUS:0142227747

VL - 3

SP - 183

EP - 189

JO - Cardiovascular Revascularization Medicine

JF - Cardiovascular Revascularization Medicine

SN - 1553-8389

IS - 3-4

ER -