Androgenic control of immunoreactive somatostatin in the Harderian gland of the Syrian hamster

M. Puig-Domingo, J. M. Guerrero, R. J. Reiter, M. A. Peinado, A. Menendez-Pelaez, C. Santana, S. M. Webb

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15 Scopus citations

Abstract

Harderian glands of Syrian hamsters contained measurable levels of immunoreactive somatostatin. After an extraction procedure, serial dilutions of tissue were assayed and showed parallelism in the displacement curve with dilutions of purified somatostatin standard in the radioimmunoassay. Somatostatin concentrations were higher in female hamsters (10.0 ± 2.1 ng/mg protein) than in males (2.6 ± 0.4 ng/mg protein). Castrated males had somatostatin values in the range of females (12.4 ± 2.3 ng/mg protein) at 1 month after gonadectomy. Testosterone implants prevented the rise of Harderian gland somatostatin in castrated males. Gonadectomized males had lower somatostatin content in the gland than did control males (1.0 ± 0.2 ng/mg protein) at 2 months after castration. Somatostatin values in females were unaffected by gonadectomy, but there were variations during the oestrous cycle, with a nadir detected at dioestrus-1 and maximal values coincident with the day of the ovulation.

Original languageEnglish (US)
Pages (from-to)753-759
Number of pages7
JournalJournal of Reproduction and Fertility
Volume82
Issue number2
DOIs
StatePublished - Jan 1 1988

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ASJC Scopus subject areas

  • Physiology
  • Embryology
  • Molecular Biology
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Puig-Domingo, M., Guerrero, J. M., Reiter, R. J., Peinado, M. A., Menendez-Pelaez, A., Santana, C., & Webb, S. M. (1988). Androgenic control of immunoreactive somatostatin in the Harderian gland of the Syrian hamster. Journal of Reproduction and Fertility, 82(2), 753-759. https://doi.org/10.1530/jrf.0.0820753