Androgen receptor in the rat brain - assays and properties

Gary C. Chamness, Timothy W. King, Peter J. Sheridan

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

The existence and relevance of an androgen receptor in the developing brain has been a matter of controversy. We here describe both sucrose density gradient and hydroxylapatite assays which clearly define a distincy androgen receptor in the 24-day-old rat hypothalamus, amygdala and preoptic region, but not in the cortex. This receptor has considerable affinity for estradiol-17ß, thus perhaps accounting for some uncertainty about its nature, but none for diethylstilbestrol or other estrogens, antiestrogens or glucocorticoids. Its Kd for both dihydrotestosterone and testosterone is about 1 × 10-9 M and for estradiol about 2 × 10-8 M. Its properties are generally consistent with those of androgen receptor reported for other tissues.

Original languageEnglish (US)
Pages (from-to)267-276
Number of pages10
JournalBrain Research
Volume161
Issue number2
DOIs
StatePublished - Feb 2 1979

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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