TY - JOUR
T1 - Anandamide levels in cerebrospinal fluid of first-episode schizophrenic patients
T2 - Impact of cannabis use
AU - Leweke, F. Markus
AU - Giuffrida, Andrea
AU - Koethe, Dagmar
AU - Schreiber, Daniela
AU - Nolden, Brit M.
AU - Kranaster, Laura
AU - Neatby, Miriam A.
AU - Schneider, Miriam
AU - Gerth, Christoph W.
AU - Hellmich, Martin
AU - Klosterkötter, Joachim
AU - Piomelli, Daniele
N1 - Funding Information:
Funding for this study was provided by the Stanley Medical Research Institute (SMRI grants 01-315 and 03-NV-003 to FML), the Koeln Fortune Program (108-2000 to FML), NARSAD (to DP), and the National Institute on Drug Abuse (DA12413 and DA12653 to DP); all funding sources had no further role in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the paper for publication. No review or approval of the manuscript was required.
PY - 2007/8
Y1 - 2007/8
N2 - Background: Previous studies have shown that cerebrospinal fluid (CSF) from schizophrenic patients contains significantly higher levels of the endogenous cannabinoid anandamide than does CSF from healthy volunteers. Moreover, CSF anandamide levels correlated inversely with psychotic symptoms, suggesting that anandamide release in the central nervous system (CNS) may serve as an adaptive mechanism countering neurotransmitter abnormalities in acute psychoses. In the present study we examined whether cannabis use may alter such a mechanism. Methods: We used liquid chromatography/mass spectrometry (LC/MS) to measure anandamide levels in serum and CSF from first-episode, antipsychotic-naïve schizophrenics (n = 47) and healthy volunteers (n = 81). Based on reported patterns of cannabis use and urine Δ9-tetrahydrocannabinol (Δ9-THC) tests, each subject group was further divided into two subgroups: 'low-frequency' and 'high-frequency' cannabis users (lifetime use ≤ 5 times and > 20 times, respectively). Serum Δ9-THC was investigated to determine acute use and three patients were excluded from the analysis due to detectable Δ9-THC levels in serum. Results: Schizophrenic low-frequency cannabis users (n = 25) exhibited > 10-fold higher CSF anandamide levels than did schizophrenic high-frequency users (n = 19, p = 0.008), healthy low-frequency (n = 55, p < 0.001) or high-frequency users (n = 26, p < 0.001). In contrast, no significant differences in serum anandamide levels were found among the four subgroups. CSF anandamide levels and disease symptoms were negatively correlated in both user groups. Conclusions: The results indicate that frequent cannabis exposure may down-regulate anandamide signaling in the CNS of schizophrenic patients, but not of healthy individuals. Thus, our findings suggest that alterations in endocannabinoid signaling might be an important component of the mechanism through which cannabis impacts mental health.
AB - Background: Previous studies have shown that cerebrospinal fluid (CSF) from schizophrenic patients contains significantly higher levels of the endogenous cannabinoid anandamide than does CSF from healthy volunteers. Moreover, CSF anandamide levels correlated inversely with psychotic symptoms, suggesting that anandamide release in the central nervous system (CNS) may serve as an adaptive mechanism countering neurotransmitter abnormalities in acute psychoses. In the present study we examined whether cannabis use may alter such a mechanism. Methods: We used liquid chromatography/mass spectrometry (LC/MS) to measure anandamide levels in serum and CSF from first-episode, antipsychotic-naïve schizophrenics (n = 47) and healthy volunteers (n = 81). Based on reported patterns of cannabis use and urine Δ9-tetrahydrocannabinol (Δ9-THC) tests, each subject group was further divided into two subgroups: 'low-frequency' and 'high-frequency' cannabis users (lifetime use ≤ 5 times and > 20 times, respectively). Serum Δ9-THC was investigated to determine acute use and three patients were excluded from the analysis due to detectable Δ9-THC levels in serum. Results: Schizophrenic low-frequency cannabis users (n = 25) exhibited > 10-fold higher CSF anandamide levels than did schizophrenic high-frequency users (n = 19, p = 0.008), healthy low-frequency (n = 55, p < 0.001) or high-frequency users (n = 26, p < 0.001). In contrast, no significant differences in serum anandamide levels were found among the four subgroups. CSF anandamide levels and disease symptoms were negatively correlated in both user groups. Conclusions: The results indicate that frequent cannabis exposure may down-regulate anandamide signaling in the CNS of schizophrenic patients, but not of healthy individuals. Thus, our findings suggest that alterations in endocannabinoid signaling might be an important component of the mechanism through which cannabis impacts mental health.
KW - Anandamide
KW - Cannabis
KW - Endocannabinoids
KW - First episode
KW - Schizophrenia
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U2 - 10.1016/j.schres.2007.04.025
DO - 10.1016/j.schres.2007.04.025
M3 - Article
C2 - 17566707
AN - SCOPUS:34447106643
VL - 94
SP - 29
EP - 36
JO - Schizophrenia Research
JF - Schizophrenia Research
SN - 0920-9964
IS - 1-3
ER -