Analytical challenges of shotgun lipidomics at different resolution of measurements

Jianing Wang, Xianlin Han

Research output: Contribution to journalReview article


The essence of shotgun lipidomics is to maintain consistency of the chemical environment of lipid samples during mass spectrometry acquisition. This strategy is suitable for large-scale quantitative analysis. This strategy also allows sufficient time to collect data to improve the signal-to-noise ratio. The initial approach of shotgun lipidomics was the electrospray ionization (ESI)-based direct infusion mass spectrometry strategy. With development of mass spectrometry for small molecules, shotgun lipidomics methods have been extended to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and ambient mass spectrometry, including MS imaging methods. Furthermore, the object of analysis has extended from organ and body fluid levels to tissue and cell levels with technological developments. In this article, we summarize the status and technical challenges of shotgun lipidomics at different resolution of measurements from the mass spectrometry perspective.

Original languageEnglish (US)
Article number115697
JournalTrAC - Trends in Analytical Chemistry
StatePublished - Dec 2019



  • High resolution mass spectrometry
  • Lipidomics
  • Mass spectrometry imaging
  • Shotgun lipidomics
  • Single-cell analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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