TY - JOUR
T1 - Analytical challenges of shotgun lipidomics at different resolution of measurements
AU - Wang, Jianing
AU - Han, Xianlin
N1 - Funding Information:
This work was partially supported by the National Institute of Health Grant RF1 AG061872 , United States, the institutional research funds from the University of Texas Health Science Center at San Antonio (UT Health SA), United States, the Mass Spectrometry Core Facility at UT Health SA, United States, and the Methodist Hospital Foundation, United States .
Funding Information:
This work was partially supported by the National Institute of Health Grant RF1 AG061872, United States, the institutional research funds from the University of Texas Health Science Center at San Antonio (UT Health SA), United States, the Mass Spectrometry Core Facility at UT Health SA, United States, and the Methodist Hospital Foundation, United States.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/12
Y1 - 2019/12
N2 - The essence of shotgun lipidomics is to maintain consistency of the chemical environment of lipid samples during mass spectrometry acquisition. This strategy is suitable for large-scale quantitative analysis. This strategy also allows sufficient time to collect data to improve the signal-to-noise ratio. The initial approach of shotgun lipidomics was the electrospray ionization (ESI)-based direct infusion mass spectrometry strategy. With development of mass spectrometry for small molecules, shotgun lipidomics methods have been extended to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and ambient mass spectrometry, including MS imaging methods. Furthermore, the object of analysis has extended from organ and body fluid levels to tissue and cell levels with technological developments. In this article, we summarize the status and technical challenges of shotgun lipidomics at different resolution of measurements from the mass spectrometry perspective.
AB - The essence of shotgun lipidomics is to maintain consistency of the chemical environment of lipid samples during mass spectrometry acquisition. This strategy is suitable for large-scale quantitative analysis. This strategy also allows sufficient time to collect data to improve the signal-to-noise ratio. The initial approach of shotgun lipidomics was the electrospray ionization (ESI)-based direct infusion mass spectrometry strategy. With development of mass spectrometry for small molecules, shotgun lipidomics methods have been extended to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and ambient mass spectrometry, including MS imaging methods. Furthermore, the object of analysis has extended from organ and body fluid levels to tissue and cell levels with technological developments. In this article, we summarize the status and technical challenges of shotgun lipidomics at different resolution of measurements from the mass spectrometry perspective.
KW - High resolution mass spectrometry
KW - Lipidomics
KW - Mass spectrometry imaging
KW - Shotgun lipidomics
KW - Single-cell analysis
UR - http://www.scopus.com/inward/record.url?scp=85073918826&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85073918826&partnerID=8YFLogxK
U2 - 10.1016/j.trac.2019.115697
DO - 10.1016/j.trac.2019.115697
M3 - Review article
C2 - 32713986
AN - SCOPUS:85073918826
SN - 0165-9936
VL - 121
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
M1 - 115697
ER -