Analysis of the Transcriptional Activity of the 5′-Flanking Region of the Rat Catalase Gene in Transiently Transfected Cells and in Transgenic Mice

Holly Van Remmen, Melissa D. Williams, Hong Yang, Christi A. Walter, Arlan Richardson

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Transiently transfected cell lines and transgenic mice were used to study the transcriptional activity of the 5′-flanking region of the catalase gene. Fragments of the 5′-flanking region of the rat catalase gene ranging in length from 3,421 base pairs (bp) to 69 bp were fused to the chloramphenicol acetyltransferase (CAT) reporter gene, and the transcriptional activity of the reporter gene was measured following transient transfection in three cell lines: a human hepatoma cell line (HepG2), a porcine kidney epithelial cell line (LLCPK1), and a human glioma cell line (U-138 MG). The 3,421-bp fragment of the 5′-flanking region resulted in a high level of expression of the reporter gene in all three cell lines. Shorter fragments of the 5′-flanking region resulted in a decrease in the level of CAT reporter expression that varied among the three cell lines, implying the presence of tissue-specific regulatory sites. To study the tissue-specific regulation of the catalase promoter, transgenic mice containing the 3,421-bp 5′-flanking sequence attached to the CAT reporter gene were produced, and CAT expression was measured in various tissues of three independent transgenic lines. CAT activity was consistently high in muscle tissue (heart, skeletal muscle, and diaphragm) and low in most other tissues studied, particularly in liver and kidney. In contrast, the endogenous expression of catalase is low in muscle and high in liver and kidney; thus, the tissue-specific expression of the reporter gene driven by the 3,421-bp fragment of the 5′-flanking region of the catalase gene was not similar to the expression of the endogenous catalase gene.

Original languageEnglish (US)
Pages (from-to)18-26
Number of pages9
JournalJournal of Cellular Physiology
Volume174
Issue number1
DOIs
StatePublished - Jan 1998

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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