Abstract
Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the δH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the δH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.
Original language | English (US) |
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Pages (from-to) | 8247-8253 |
Number of pages | 7 |
Journal | Journal of virology |
Volume | 87 |
Issue number | 14 |
DOIs | |
State | Published - Jul 2013 |
ASJC Scopus subject areas
- Insect Science
- Virology
- Microbiology
- Immunology