Abstract
Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the δH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the δH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8247-8253 |
| Number of pages | 7 |
| Journal | Journal of Virology |
| Volume | 87 |
| Issue number | 14 |
| DOIs | |
| State | Published - Jul 2013 |
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ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant. / Meng, Xiangzhi; Wu, Xiang; Yan, Bo; Deng, Junpeng; Xiang, Yan.
In: Journal of Virology, Vol. 87, No. 14, 07.2013, p. 8247-8253.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant
AU - Meng, Xiangzhi
AU - Wu, Xiang
AU - Yan, Bo
AU - Deng, Junpeng
AU - Xiang, Yan
PY - 2013/7
Y1 - 2013/7
N2 - Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the δH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the δH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.
AB - Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the δH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the δH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.
UR - http://www.scopus.com/inward/record.url?scp=84880302581&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880302581&partnerID=8YFLogxK
U2 - 10.1128/JVI.00845-13
DO - 10.1128/JVI.00845-13
M3 - Article
C2 - 23678177
AN - SCOPUS:84880302581
VL - 87
SP - 8247
EP - 8253
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 14
ER -