Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant

Xiangzhi Meng; Xiang Wu; Bo Yan; Junpeng Deng; Yan Xiang

doi:10.1128/JVI.00845-13

Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant

Xiangzhi Meng, Xiang Wu, Bo Yan, Junpeng Deng, Yan Xiang

Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the δH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the δH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.

Original language	English (US)
Pages (from-to)	8247-8253
Number of pages	7
Journal	Journal of virology
Volume	87
Issue number	14
DOIs	https://doi.org/10.1128/JVI.00845-13
State	Published - Jul 2013

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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abstract = "Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the δH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the δH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.",

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AU - Meng, Xiangzhi

AU - Wu, Xiang

AU - Yan, Bo

AU - Deng, Junpeng

AU - Xiang, Yan

PY - 2013/7

Y1 - 2013/7

N2 - Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the δH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the δH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.

AB - Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the δH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the δH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.

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Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant

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