Analysis of the regulation of lin-41 during chick and mouse limb development

Joseph J. Lancman, Nicholas C. Caruccio, Brian D. Harfe, Amy E. Pasquinelli, Jeoffrey J. Schageman, Alexander Pertsemlidis, John F. Fallon

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We have cloned the chicken and mouse orthologues of the Caenorhabditis elegans heterochronic gene lin-41. During limb development, lin-41 is expressed in three phases over developmental time and most notably is associated with the developing autopod. Using chicken and mouse mutants and bead implantations, we report that lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways. In C. elegans, it is proposed that lin-41 activity is temporally regulated by miRNAs (let-7 and lin-4) that bind to complementary sites in the lin-41 3'-untranslated region (UTR). Taking a bioinformatics approach, we also report the presence of potential miRNA binding sites in the 3'-UTR of chicken lin-41, including sites for the chicken orthologues of both C. elegans let-7 and lin-4. Finally, we show that these miRNAs and others are expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin-41 activity in vivo.

Original languageEnglish (US)
Pages (from-to)948-960
Number of pages13
JournalDevelopmental Dynamics
Volume234
Issue number4
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Chickens
Extremities
Caenorhabditis elegans
MicroRNAs
3' Untranslated Regions
Computational Biology
Binding Sites
Genes

Keywords

  • Developmental timing
  • Fgf
  • Heterochronic gene
  • Let-7
  • Limb development
  • Lin-41
  • miR-125
  • miRNA
  • Muscle development
  • Pattern formation
  • Shh
  • Sonic Hedgehog
  • Vascular development

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Lancman, J. J., Caruccio, N. C., Harfe, B. D., Pasquinelli, A. E., Schageman, J. J., Pertsemlidis, A., & Fallon, J. F. (2005). Analysis of the regulation of lin-41 during chick and mouse limb development. Developmental Dynamics, 234(4), 948-960. https://doi.org/10.1002/dvdy.20591

Analysis of the regulation of lin-41 during chick and mouse limb development. / Lancman, Joseph J.; Caruccio, Nicholas C.; Harfe, Brian D.; Pasquinelli, Amy E.; Schageman, Jeoffrey J.; Pertsemlidis, Alexander; Fallon, John F.

In: Developmental Dynamics, Vol. 234, No. 4, 12.2005, p. 948-960.

Research output: Contribution to journalArticle

Lancman, JJ, Caruccio, NC, Harfe, BD, Pasquinelli, AE, Schageman, JJ, Pertsemlidis, A & Fallon, JF 2005, 'Analysis of the regulation of lin-41 during chick and mouse limb development', Developmental Dynamics, vol. 234, no. 4, pp. 948-960. https://doi.org/10.1002/dvdy.20591
Lancman JJ, Caruccio NC, Harfe BD, Pasquinelli AE, Schageman JJ, Pertsemlidis A et al. Analysis of the regulation of lin-41 during chick and mouse limb development. Developmental Dynamics. 2005 Dec;234(4):948-960. https://doi.org/10.1002/dvdy.20591
Lancman, Joseph J. ; Caruccio, Nicholas C. ; Harfe, Brian D. ; Pasquinelli, Amy E. ; Schageman, Jeoffrey J. ; Pertsemlidis, Alexander ; Fallon, John F. / Analysis of the regulation of lin-41 during chick and mouse limb development. In: Developmental Dynamics. 2005 ; Vol. 234, No. 4. pp. 948-960.
@article{2758329603a24f25aebb537588bcda16,
title = "Analysis of the regulation of lin-41 during chick and mouse limb development",
abstract = "We have cloned the chicken and mouse orthologues of the Caenorhabditis elegans heterochronic gene lin-41. During limb development, lin-41 is expressed in three phases over developmental time and most notably is associated with the developing autopod. Using chicken and mouse mutants and bead implantations, we report that lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways. In C. elegans, it is proposed that lin-41 activity is temporally regulated by miRNAs (let-7 and lin-4) that bind to complementary sites in the lin-41 3'-untranslated region (UTR). Taking a bioinformatics approach, we also report the presence of potential miRNA binding sites in the 3'-UTR of chicken lin-41, including sites for the chicken orthologues of both C. elegans let-7 and lin-4. Finally, we show that these miRNAs and others are expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin-41 activity in vivo.",
keywords = "Developmental timing, Fgf, Heterochronic gene, Let-7, Limb development, Lin-41, miR-125, miRNA, Muscle development, Pattern formation, Shh, Sonic Hedgehog, Vascular development",
author = "Lancman, {Joseph J.} and Caruccio, {Nicholas C.} and Harfe, {Brian D.} and Pasquinelli, {Amy E.} and Schageman, {Jeoffrey J.} and Alexander Pertsemlidis and Fallon, {John F.}",
year = "2005",
month = "12",
doi = "10.1002/dvdy.20591",
language = "English (US)",
volume = "234",
pages = "948--960",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Analysis of the regulation of lin-41 during chick and mouse limb development

AU - Lancman, Joseph J.

AU - Caruccio, Nicholas C.

AU - Harfe, Brian D.

AU - Pasquinelli, Amy E.

AU - Schageman, Jeoffrey J.

AU - Pertsemlidis, Alexander

AU - Fallon, John F.

PY - 2005/12

Y1 - 2005/12

N2 - We have cloned the chicken and mouse orthologues of the Caenorhabditis elegans heterochronic gene lin-41. During limb development, lin-41 is expressed in three phases over developmental time and most notably is associated with the developing autopod. Using chicken and mouse mutants and bead implantations, we report that lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways. In C. elegans, it is proposed that lin-41 activity is temporally regulated by miRNAs (let-7 and lin-4) that bind to complementary sites in the lin-41 3'-untranslated region (UTR). Taking a bioinformatics approach, we also report the presence of potential miRNA binding sites in the 3'-UTR of chicken lin-41, including sites for the chicken orthologues of both C. elegans let-7 and lin-4. Finally, we show that these miRNAs and others are expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin-41 activity in vivo.

AB - We have cloned the chicken and mouse orthologues of the Caenorhabditis elegans heterochronic gene lin-41. During limb development, lin-41 is expressed in three phases over developmental time and most notably is associated with the developing autopod. Using chicken and mouse mutants and bead implantations, we report that lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways. In C. elegans, it is proposed that lin-41 activity is temporally regulated by miRNAs (let-7 and lin-4) that bind to complementary sites in the lin-41 3'-untranslated region (UTR). Taking a bioinformatics approach, we also report the presence of potential miRNA binding sites in the 3'-UTR of chicken lin-41, including sites for the chicken orthologues of both C. elegans let-7 and lin-4. Finally, we show that these miRNAs and others are expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin-41 activity in vivo.

KW - Developmental timing

KW - Fgf

KW - Heterochronic gene

KW - Let-7

KW - Limb development

KW - Lin-41

KW - miR-125

KW - miRNA

KW - Muscle development

KW - Pattern formation

KW - Shh

KW - Sonic Hedgehog

KW - Vascular development

UR - http://www.scopus.com/inward/record.url?scp=28444491759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28444491759&partnerID=8YFLogxK

U2 - 10.1002/dvdy.20591

DO - 10.1002/dvdy.20591

M3 - Article

C2 - 16245339

AN - SCOPUS:28444491759

VL - 234

SP - 948

EP - 960

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 4

ER -