Analysis of the regulation of lin-41 during chick and mouse limb development

Joseph J. Lancman, Nicholas C. Caruccio, Brian D. Harfe, Amy E. Pasquinelli, Jeoffrey J. Schageman, Alexander Pertsemlidis, John F. Fallon

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


We have cloned the chicken and mouse orthologues of the Caenorhabditis elegans heterochronic gene lin-41. During limb development, lin-41 is expressed in three phases over developmental time and most notably is associated with the developing autopod. Using chicken and mouse mutants and bead implantations, we report that lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways. In C. elegans, it is proposed that lin-41 activity is temporally regulated by miRNAs (let-7 and lin-4) that bind to complementary sites in the lin-41 3'-untranslated region (UTR). Taking a bioinformatics approach, we also report the presence of potential miRNA binding sites in the 3'-UTR of chicken lin-41, including sites for the chicken orthologues of both C. elegans let-7 and lin-4. Finally, we show that these miRNAs and others are expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin-41 activity in vivo.

Original languageEnglish (US)
Pages (from-to)948-960
Number of pages13
JournalDevelopmental Dynamics
Issue number4
StatePublished - Dec 2005
Externally publishedYes


  • Developmental timing
  • Fgf
  • Heterochronic gene
  • Let-7
  • Limb development
  • Lin-41
  • Muscle development
  • Pattern formation
  • Shh
  • Sonic Hedgehog
  • Vascular development
  • miR-125
  • miRNA

ASJC Scopus subject areas

  • Developmental Biology


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