Analysis of the daily changes of melatonin receptors in the rat liver

Carmen Venegas, José A. García, Carolina Doerrier, Huayqui Volt, Germaine Escames, Luis C. Lõpez, Russel J Reiter, Darío Acuña-Castroviejo

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Melatonin membrane (MT1 and MT2) and nuclear (RORα) receptors have been identified in several mammalian tissues, including the liver. The mechanisms regulating hepatic melatonin receptors are yet unknown. This study investigated whether these receptors exhibit daily changes and the effects of melatonin on their levels. Our results show that mRNAs for MT1/MT2 receptors exhibit circadian rhythms that were followed by rhythms in their respective protein levels; the acrophases for the two rhythms were reached at 04:00 and 05:00 hr, respectively. Pinealectomy blunted the rhythms in both mRNAs and protein levels. In contrast, mRNA and protein levels of nuclear receptor RORα increased significantly after pinealectomy. The cycles of the latter receptor also exhibited circadian rhythms which peaked at 03:00 and 03:45 hr, respectively. Melatonin administration (10-200 mg/kg) increased in a dose-dependent manner the protein content of MT1/MT2 receptors, with no effects on RORα. Lunzindole treatment, however, did not affect melatonin receptor expression or content of either the membrane or nuclear receptors. Together with previously published findings which demonstrated the intracellular distribution of melatonin in rat liver, the current results support the conclusion that the circadian rhythms of MT1/MT2 and RORα receptors are under the control of the serum and intracellular melatonin levels. Moreover, the induction of MT1/MT2 receptors after the administration of high doses of melatonin further suggests that the therapeutic value of melatonin may not be restricted to only low doses of the indoleamine.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalJournal of Pineal Research
Volume54
Issue number3
DOIs
StatePublished - Apr 2013

Fingerprint

Melatonin Receptors
Melatonin
Melatonin MT2 Receptor
Melatonin MT1 Receptor
Liver
Cytoplasmic and Nuclear Receptors
Circadian Rhythm
Messenger RNA
Proteins
Membranes
Serum

Keywords

  • circadian rhythms
  • melatonin
  • MT1
  • MT2
  • rat liver
  • RORα

ASJC Scopus subject areas

  • Endocrinology

Cite this

Venegas, C., García, J. A., Doerrier, C., Volt, H., Escames, G., Lõpez, L. C., ... Acuña-Castroviejo, D. (2013). Analysis of the daily changes of melatonin receptors in the rat liver. Journal of Pineal Research, 54(3), 313-321. https://doi.org/10.1111/jpi.12019

Analysis of the daily changes of melatonin receptors in the rat liver. / Venegas, Carmen; García, José A.; Doerrier, Carolina; Volt, Huayqui; Escames, Germaine; Lõpez, Luis C.; Reiter, Russel J; Acuña-Castroviejo, Darío.

In: Journal of Pineal Research, Vol. 54, No. 3, 04.2013, p. 313-321.

Research output: Contribution to journalArticle

Venegas, C, García, JA, Doerrier, C, Volt, H, Escames, G, Lõpez, LC, Reiter, RJ & Acuña-Castroviejo, D 2013, 'Analysis of the daily changes of melatonin receptors in the rat liver', Journal of Pineal Research, vol. 54, no. 3, pp. 313-321. https://doi.org/10.1111/jpi.12019
Venegas C, García JA, Doerrier C, Volt H, Escames G, Lõpez LC et al. Analysis of the daily changes of melatonin receptors in the rat liver. Journal of Pineal Research. 2013 Apr;54(3):313-321. https://doi.org/10.1111/jpi.12019
Venegas, Carmen ; García, José A. ; Doerrier, Carolina ; Volt, Huayqui ; Escames, Germaine ; Lõpez, Luis C. ; Reiter, Russel J ; Acuña-Castroviejo, Darío. / Analysis of the daily changes of melatonin receptors in the rat liver. In: Journal of Pineal Research. 2013 ; Vol. 54, No. 3. pp. 313-321.
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