Analysis of serum total and free PSA using immunoaffinity depletion coupled to SRM: Correlation with clinical immunoassay tests

Tao Liu, Mahmud Hossain, Athena A. Schepmoes, Thomas L. Fillmore, Lori J. Sokoll, Scott R. Kronewitter, Grant Izmirlian, Tujin Shi, Wei Jun Qian, Robin J. Leach, Ian M. Thompson, Daniel W. Chan, Richard D. Smith, Jacob Kagan, Sudhir Srivastava, Karin D. Rodland, David G. Camp

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Recently, selected reaction monitoring mass spectrometry (SRM-MS) has been more frequently applied to measure low abundance biomarker candidates in tissues and biofluids, owing to its high sensitivity and specificity, simplicity of assay configuration, and exceptional multiplexing capability. In this study, we report for the first time the development of immunoaffinity depletion-based workflows and SRM-MS assays that enable sensitive and accurate quantification of total and free prostate-specific antigen (PSA) in serum without the requirement for specific PSA antibodies. Low ng/mL level detection of both total and free PSA was consistently achieved in both PSA-spiked female serum samples and actual patient serum samples. Moreover, comparison of the results obtained when SRM PSA assays and conventional immunoassays were applied to the same samples showed good correlation in several independent clinical serum sample sets. These results demonstrate that the workflows and SRM assays developed here provide an attractive alternative for reliably measuring candidate biomarkers in human blood, without the need to develop affinity reagents. Furthermore, the simultaneous measurement of multiple biomarkers, including the free and bound forms of PSA, can be performed in a single multiplexed analysis using high-resolution liquid chromatographic separation coupled with SRM-MS. This article is part of a Special Issue entitled: Translational Proteomics.

Original languageEnglish (US)
Pages (from-to)4747-4757
Number of pages11
JournalJournal of Proteomics
Volume75
Issue number15
DOIs
StatePublished - Aug 3 2012

Keywords

  • Free PSA
  • Immunoaffinity depletion
  • Immunoassay
  • Selected reaction monitoring
  • Serum
  • Total PSA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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    Liu, T., Hossain, M., Schepmoes, A. A., Fillmore, T. L., Sokoll, L. J., Kronewitter, S. R., Izmirlian, G., Shi, T., Qian, W. J., Leach, R. J., Thompson, I. M., Chan, D. W., Smith, R. D., Kagan, J., Srivastava, S., Rodland, K. D., & Camp, D. G. (2012). Analysis of serum total and free PSA using immunoaffinity depletion coupled to SRM: Correlation with clinical immunoassay tests. Journal of Proteomics, 75(15), 4747-4757. https://doi.org/10.1016/j.jprot.2012.01.035