Analysis of ribonucleotides and deoxyribonucleotides using high-pressure liquid column chromatography

W. P. Kennedy, J. C. Lee

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Conditions for the separation of some mononucleotides, dinucleotides, nucleoside diphosphates and deoxymononucleotides on a pellicular anion-exchange column are described. These chromatographic systems allow quantification and identification of nucleotide materials in nanomole quantities and can be achieved within 100 min. The applicability of the present systems to the analysis of base composition of polynucleotides has been demonstrated by analyzing some oligonucleotides of known structure.

Original languageEnglish (US)
Pages (from-to)203-209
Number of pages7
JournalJournal of Chromatography A
Volume51
Issue numberC
DOIs
StatePublished - 1970

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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