Analysis of rat hindlimb muscle proteins by two-dimensional gels following spinal cord injury

John C. Lee, Angela M. Braun

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Proteins from extensor digitorum longus (EDL), plantaris (PLN), and soleus (SOL) muscles of adult, female rats were examined by high resolution two-dimensional gel electrophoresis up to 4 weeks following spinal cord transection. The electrophoretograms were analyzed by computer imaging and densitometry. Reproducible and significant changes in the relative concentrations of several proteins in each muscle type were detected. Whereas changes involving the largest number of proteins were observed in SOL, changes in EDL and PLN were also detected. In SOL, approximately 50% of the altered proteins increased in concentration and the remaining decreased: Actin and myosin light chains LCF-1 and LCF-2 were among those proteins whose concentrations increased, whereas myosin light chains LCS-1 and LCS-2 were among those proteins whose concentrations decreased. The present findings regarding the reversal in myosin light chain composition provide biochemical support for previously published data on changes in contractile properties of muscles following spinalization. In EDL, the relative concentration of only one protein was elevated in a time-dependent manner. The concentrations of two protein species in PLN were increased following cord transection. These findings provide new biochemical markers on the effects of spinal cord on gene expression in specific hindlimb skeletal muscles.

Original languageEnglish (US)
Pages (from-to)96-101
Number of pages6
JournalJournal of Neurochemistry
Volume54
Issue number1
StatePublished - 1990

Fingerprint

Muscle Proteins
Hindlimb
Spinal Cord Injuries
Rats
Gels
Myosin Light Chains
Muscle
Proteins
Skeletal Muscle
Muscles
Densitometry
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Gene expression
Actins
Spinal Cord
Biomarkers
Gene Expression
Imaging techniques
Chemical analysis

Keywords

  • Muscle proteins
  • Spinal cord injury
  • Two-dimensional gels

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Analysis of rat hindlimb muscle proteins by two-dimensional gels following spinal cord injury. / Lee, John C.; Braun, Angela M.

In: Journal of Neurochemistry, Vol. 54, No. 1, 1990, p. 96-101.

Research output: Contribution to journalArticle

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