Analysis of possible abnormal WT1 RNA processing in primary Wilms tumors

Kerry B. Gunning, Susan L. Cohn, Gail E. Tomlinson, Louise C. Strong, Vicki Huff

Research output: Contribution to journalArticle

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Abstract

WT1 RNA processing abnormalities have been suggested to play a role in the development of Wilms tumor by reports of editing at codon 280 in the rat WT1 transcript (codon 281 in humans) and aberrant splicing of exon 2 in WT1 transcripts from Wilms tumor xenograft cell lines, Both events result in a functionally changed WT1 protein and are potential mechanisms of altering normal protein function in the absence of WT1 DNA mutations. To determine whether either of these RNA processing events occurs in primary Wilms tumors, we analysed WT1 mRNA from 15 primary tumors. There was no evidence of WT1 RNA editing at codon 281, and only one primary tumor displayed aberrant splicing of exon 2. Sequence and Southern analysis of DNA from this tumor did not reveal any alteration in or around exon 2. These results suggest that neither RNA editing at codon 281 nor abberant exon 2 splicing is a frequent mechanism of WT1 alteration during tumorigenesis.

Original languageEnglish (US)
Pages (from-to)1179-1185
Number of pages7
JournalOncogene
Volume13
Issue number6
Publication statusPublished - Jan 1 1996

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Keywords

  • Aberrant splicing
  • Editing
  • RNA
  • WT1
  • Wilms tumor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Gunning, K. B., Cohn, S. L., Tomlinson, G. E., Strong, L. C., & Huff, V. (1996). Analysis of possible abnormal WT1 RNA processing in primary Wilms tumors. Oncogene, 13(6), 1179-1185.