Analysis of neuraminidase isozyme phenotypes in mammalian tissues: An electrophoretic approach

Paul B. Samollow, John L. VandeBerg, Heinz W. Kunz, Thomas J. Gill

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A simple cellulose acetate electrophoretic method for visualizing mammalian neuraminidase isozymes has been developed. Application of the method with rat and mouse liver extracts reveals the presence of two distinct isozymes in each species. Each isozyme exhibits tremendous variation in activity between inbred strains. The two isozymes vary independently of one another suggesting that their activities are controlled by different genes. The neuraminidase phenotypes detected in these inbred strains via electrophoresis are consistent with published accounts of neuraminidase phenotypes determined fluorometrically in whole liver homogenates, but also indicate the presence of a second isozyme not perceived by this other procedure.

Original languageEnglish (US)
Pages (from-to)1182-1188
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume126
Issue number3
DOIs
StatePublished - Feb 15 1985
Externally publishedYes

Fingerprint

Neuraminidase
Isoenzymes
Tissue
Phenotype
Liver Extracts
Electrophoresis
Liver
Rats
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Analysis of neuraminidase isozyme phenotypes in mammalian tissues : An electrophoretic approach. / Samollow, Paul B.; VandeBerg, John L.; Kunz, Heinz W.; Gill, Thomas J.

In: Biochemical and Biophysical Research Communications, Vol. 126, No. 3, 15.02.1985, p. 1182-1188.

Research output: Contribution to journalArticle

Samollow, Paul B. ; VandeBerg, John L. ; Kunz, Heinz W. ; Gill, Thomas J. / Analysis of neuraminidase isozyme phenotypes in mammalian tissues : An electrophoretic approach. In: Biochemical and Biophysical Research Communications. 1985 ; Vol. 126, No. 3. pp. 1182-1188.
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