Analysis of MYC bound loci identified by CpG island arrays shows that Max is essential for MYC-dependent repression

Daniel Y L Mao, John D. Watson, Pearlly S. Yan, Dalia Barsyte-Lovejoy, Fereshteh Khosravi, W. Wei-Lynn Wong, Peggy J. Farnham, Hui-ming Huang, Linda Z. Penn

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

The c-myc proto-oncogene encodes a transcription factor, c-Myc, which is deregulated and/or overexpressed in many human cancers. Despite c-Myc's importance, the identity of Myc-regulated genes and the mechanism by which Myc regulates these genes remain unclear. By combining chromatin immunoprecipitation with CpG island arrays, we identified 177 human genomic loci that are bound by Myc in vivo. Analyzing a cohort of known and novel Myc target genes showed that Myc-associated protein X, Max, also bound to these regulatory regions. Indeed, Max is bound to these loci in the presence or absence of Myc. The Myc:Max interaction is essential for Myc-dependent transcriptional activation; however, we show that Max bound targets also include Myc-repressed genes. Moreover, we show that the interaction between Myc and Max is essential for gene repression to occur. Taken together, the identification and analysis of Myc bound target genes supports a model whereby Max plays an essential and universal role in the mechanism of Myc-dependent transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)882-886
Number of pages5
JournalCurrent Biology
Volume13
Issue number10
DOIs
StatePublished - May 13 2003
Externally publishedYes

Fingerprint

CpG Islands
myc Genes
Genes
loci
genes
proto-oncogenes
Chromatin Immunoprecipitation
Nucleic Acid Regulatory Sequences
Essential Genes
transcriptional activation
Transcriptional Activation
chromatin
Transcription Factors
transcription factors
Chromatin
genomics
Chemical activation
neoplasms
Neoplasms
proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Mao, D. Y. L., Watson, J. D., Yan, P. S., Barsyte-Lovejoy, D., Khosravi, F., Wei-Lynn Wong, W., ... Penn, L. Z. (2003). Analysis of MYC bound loci identified by CpG island arrays shows that Max is essential for MYC-dependent repression. Current Biology, 13(10), 882-886. https://doi.org/10.1016/S0960-9822(03)00297-5

Analysis of MYC bound loci identified by CpG island arrays shows that Max is essential for MYC-dependent repression. / Mao, Daniel Y L; Watson, John D.; Yan, Pearlly S.; Barsyte-Lovejoy, Dalia; Khosravi, Fereshteh; Wei-Lynn Wong, W.; Farnham, Peggy J.; Huang, Hui-ming; Penn, Linda Z.

In: Current Biology, Vol. 13, No. 10, 13.05.2003, p. 882-886.

Research output: Contribution to journalArticle

Mao, DYL, Watson, JD, Yan, PS, Barsyte-Lovejoy, D, Khosravi, F, Wei-Lynn Wong, W, Farnham, PJ, Huang, H & Penn, LZ 2003, 'Analysis of MYC bound loci identified by CpG island arrays shows that Max is essential for MYC-dependent repression', Current Biology, vol. 13, no. 10, pp. 882-886. https://doi.org/10.1016/S0960-9822(03)00297-5
Mao, Daniel Y L ; Watson, John D. ; Yan, Pearlly S. ; Barsyte-Lovejoy, Dalia ; Khosravi, Fereshteh ; Wei-Lynn Wong, W. ; Farnham, Peggy J. ; Huang, Hui-ming ; Penn, Linda Z. / Analysis of MYC bound loci identified by CpG island arrays shows that Max is essential for MYC-dependent repression. In: Current Biology. 2003 ; Vol. 13, No. 10. pp. 882-886.
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