Analysis of mitochondrial electron transport complexes by MALDI-TOF mass spectrometry

Susan T. Weintraub, Andrej Musatov, Christopher A. Carroll, Neal C. Robinson

Research output: Contribution to conferencePaper

Abstract

The mitochondrial electron transport complexes were analyzed using MALDI-TOF mass spectrometry (MS) molecular masses of the complexes, cytochrome c oxidase (CcO) and cytochrome bc 1, were determined for the investigation on the oxidative damage to electron transport proteins. Prior to MS analysis, DM was exchanged for the detergent in the protein samples by dialysis. The addition of DM to solution of CcO and Cytochrome bc 1 was found to aid in detection of all subunits of the complex proteins.

Original languageEnglish (US)
Pages205-206
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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