Analysis of mitochondrial DNA mutations in D-loop region in thyroid lesions

Zhinan Ding, Jingzhang Ji, Guorong Chen, Hezhi Fang, Shihui Yan, Lijun Shen, Jia Wei, Kaiyan Yang, Jianxin Lu, Yidong Bai

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Background: Mitochondrial defects have been associated with various human conditions including cancers. Methods: We analyzed the mutations at the mitochondrial DNA (mtDNA) in patients with different thyroid lesions. In particular, in order to investigate if the accumulation of mtDNA mutations play a role in tumor progression, we studied the highly variable main control region of mtDNA, the displacement-loop (D-loop) in patients with non-tumor nodular goiters, with benign thyroid adenomas, and with malignant thyroid carcinomas. Total thyroid tumor or goiter samples were obtained from 101 patients, matched with nearby normal tissue and blood from the same subject. Results: Noticeably, mitochondrial microsatellite instability (mtMSI) was detected in 2 of 19 nodular goiters (10.53%), and 8 of 77 (10.39%) malignant thyroid carcinomas. In addition, 6 patients, including 5 (6.49%) with malignant thyroid carcinomas and 1 (5.26%) with nodular goiter, were found to harbor point mutations. The majority of the mutations detected were heteroplasmic. General significance: Our results indicate that mtDNA alterations in the D-loop region could happen before tumorigenesis in thyroid, and they might also accumulate during tumorigenesis.

Original languageEnglish (US)
Pages (from-to)271-274
Number of pages4
JournalBiochimica et Biophysica Acta - General Subjects
Volume1800
Issue number3
DOIs
StatePublished - Mar 1 2010

Keywords

  • D-loop region
  • Mitochondrial DNA mutation
  • Mitochondrial microsatellite instability
  • Thyroid
  • Tumor progression

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Analysis of mitochondrial DNA mutations in D-loop region in thyroid lesions'. Together they form a unique fingerprint.

Cite this