Analysis of helper-T-cell function by acetylcholine receptor-reactive cell lines of defined AChR-subunit specificity

Keith A. Krolick, Olgui E. Urso

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

A small panel of cloned acetylcholine receptor (AChR)-reactive helper T cells were examined for their ability to proliferate when stimulated with AChR or purified AChR subunits. It was observed that all T-cell lines preferentially responded to the AChR α subunit, but that some also were stimulated by other subunits as well. This was interpreted as indicating that the α subunit plays a major role in anti-AChR T-cell responses, but that considerable potential cross-reactivity exists among the subunits recognized by T cells. Furthermore, a high level of "microheterogeneity" in the T-cell-specificity repertoire was suggested by the fact that even this small panel of cloned lines expressed several patterns of subunit reactivity. Finally, all cloned T-cell lines examined were found to be capable of functioning as regulatory helpers in vitro by providing the necessary signals to AChR-responsive B cells, resulting in anti-AChR antibody production.

Original languageEnglish (US)
Pages (from-to)75-85
Number of pages11
JournalCellular Immunology
Volume105
Issue number1
DOIs
StatePublished - Mar 1987

ASJC Scopus subject areas

  • Immunology

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