Analysis of effect of age on synthesis of specific proteins by hepatocytes

J. A. Butler, A. R. Heydari, A. Richardson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The effect of age on the synthesis of specific proteins by hepatocytes was studied in Fischer F344 rats using two-dimensional polyacrylamide gel electrophoresis. Almost all proteins synthesized by hepatocytes from young rats were synthesized by hepatocytes isolated from old rats. Of over 500 proteins visually compared by two-dimensional polyacrylamide gel electrophoresis, only 11 proteins were observed to disappear and/or appear consistently with increasing age. The rates of synthesis of 36 randomly chosen proteins were quantified. Interestingly, the synthesis of 35 of the 36 proteins decreased btween 5 and 30 months of age. The decrease in protein synthesis varied (15% to 70%) from one protein to another; i.e., a heterogeneity was observed in the age-related decrease in the synthesis of proteins. The age-related decrease in protien synthesis was statistically significant for 53% of the proteins studied. The total decrease in the rate of synthesis of all 36 proteins studied was 40% between 5 and 30 months of age, which is essentially the same as the decrease in total protein synthesis by suspension of hepatocytes isolated from 5- and 30-month-old rats. The results of this study demonstrate that the mechanism underlying aging is different from development, which is characterized by a major change in the species of protein, synthesized by a cell.

Original languageEnglish (US)
Pages (from-to)400-409
Number of pages10
JournalJournal of Cellular Physiology
Volume141
Issue number2
StatePublished - 1989
Externally publishedYes

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Hepatocytes
Proteins
Rats
Inbred F344 Rats
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Suspensions
Aging of materials

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Butler, J. A., Heydari, A. R., & Richardson, A. (1989). Analysis of effect of age on synthesis of specific proteins by hepatocytes. Journal of Cellular Physiology, 141(2), 400-409.

Analysis of effect of age on synthesis of specific proteins by hepatocytes. / Butler, J. A.; Heydari, A. R.; Richardson, A.

In: Journal of Cellular Physiology, Vol. 141, No. 2, 1989, p. 400-409.

Research output: Contribution to journalArticle

Butler, JA, Heydari, AR & Richardson, A 1989, 'Analysis of effect of age on synthesis of specific proteins by hepatocytes', Journal of Cellular Physiology, vol. 141, no. 2, pp. 400-409.
Butler, J. A. ; Heydari, A. R. ; Richardson, A. / Analysis of effect of age on synthesis of specific proteins by hepatocytes. In: Journal of Cellular Physiology. 1989 ; Vol. 141, No. 2. pp. 400-409.
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