Analysis of detergent-solubilized membrane proteins in the analytical ultracentrifuge

Neal C. Robinson, Baltazar Gomez, Andrej Musatov, Jaime Ortega-Lopez

Research output: Contribution to journalReview articlepeer-review

12 Scopus citations


Hydrodynamic analysis of detergent-solubilized membrane proteins and protein complexes in the analytical ultracentrifuge is similar in principle to that of normal soluble proteins. However, caution must be exercised in choosing an appropriate solubilizing detergent and in interpreting the data. Accurate protein molecular weights can be obtained and self-association can be studied, but proper corrections for bound detergent are essential. However, it is usually not possible to obtain useful information of protein size or shape since the frictional coefficient is often dominated by the hydrodynamic size of the bound detergent.

Original languageEnglish (US)
Pages (from-to)960-968
Number of pages9
Issue number13
StatePublished - 1998

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Molecular Biology


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