Analysis of biological motors via multidimensional fractionation: A strategy

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Past strategies for the analysis of ATP-fueled motors include single-motor analysis. Single-motor analysis bypasses limitations caused by motor asynchrony during the traditional ensemble averaging analysis. The present communication describes revised ensemble averaging analysis that also can bypass asynchrony-derived limitations. This revised analysis makes measurements of one motor variable dependent on the others. One example is nondenaturing gel electrophoresis with more than one dimension. Each dimension measures one of the motor variables. This multidimensional procedure is used to obtain the values of "conformational" motor variables as a function of a "clock" motor variable. In theory, the cycle of the motor can be analyzed from a single multidimensional analysis of a collection of asynchronous motors sampled at only one time. That is to say, motor asynchrony becomes an asset, rather than a liability.

Original languageEnglish (US)
Pages (from-to)494-499
Number of pages6
JournalELECTROPHORESIS
Volume26
Issue number2
DOIs
StatePublished - Jan 2005

Keywords

  • Agarose gel electrophoresis
  • Bacteriophage assembly
  • Single-molecule analysis
  • Size determination
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Analysis of biological motors via multidimensional fractionation: A strategy'. Together they form a unique fingerprint.

Cite this