Analysis of a large nontranscribed spacer in the ribosomal DNA of the house cricket, Acheta domesticus (Orthoptera:Gryllidae)

Z. Dave Sharp, Anna Meriwether, Jerry Ware, M. Donald Cave

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

An analysis of a 29-kilobase nontranscribed spacer fragment in the ribosomal DNA (rDNA) of the house cricket, Acheta domesticus, revealed a highly repetitious structure. A total of eight EcoRI repeats of three different size classes measuring 259, 420, and 508 base pairs (bp) was mapped to a region 2 kilobases (kb) from the 18 S coding region. The repeats were oriented in a nonrandom manner and had sequences homologous to DNA located immediately adjacent to the repetitive array. DNA sequence analysis showed that the reptitive region was composed of smaller direct repeats 66, 67, and 383 bp in length. There was minor length heterogeneity of the chromosomal restriction fragments containing the entire array, indicating that a variable number of EcoRI repeats is a minor contributor to the total repeat-unit length heterogeneity. Immediately upstream from the EcoRI array there is a 17-kb region composed of 50 to 60 subrepeat elements recognized by a variety of restriction endonucleases. A subcloned SmaI repeat from the array was not homologous to any other part of the rDNA repeat unit or other chromosomal DNA. There was little length heterogeneity in restriction fragments containing the chromosomal 17-kb repetitions region. Immediately upstream from the 17-Kb region there is a 4.1-kb segment with sequences homologous to the EcoRI repeats.

Original languageEnglish (US)
Pages (from-to)911-923
Number of pages13
JournalBiochemical Genetics
Volume24
Issue number11-12
DOIs
StatePublished - Dec 1 1986

    Fingerprint

Keywords

  • direct repeats
  • nontranscribed spacer
  • ribosomal DNA (rDNA)
  • sequence homologies

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this