An unusual expression of a squamous cell marker, small proline-rich protein gene, in tracheobronchial epithelium: differential regulation and gene mapping.

G. An, Hui-ming Huang, J. Tesfaigzi, J. Garcia-Heras, D. H. Ledbetter, D. M. Carlson, R. Wu

Research output: Contribution to journalArticle

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Abstract

An unusual expression of a putative squamous cell marker, small proline-rich protein (spr1), in mucociliary epithelial cells of conducting airways was demonstrated in a serum-free culture system. A cDNA clone was isolated from the cDNA library of monkey tracheobronchial epithelial (TBE) cells by differential hybridization. This cDNA clone, MT5, exhibited 98% homology to a DNA sequence obtained from human keratinocytes treated with either UV light or phorbol esters (T. Kartasova et al., 1988, Mol. Cell. Biol. 8:2195-2230). The predicted peptide of MT5 is unusual for its high content of proline (29%), glutamine (18%), and cysteine (9%) and its repeated PKVPEPC units. The level of spr1 mRNA in cultured cells was inhibited more than 90% by vitamin A. In contrast, phorbol 12-myristate 13-acetate (PMA) stimulated the level of spr1 mRNA by 3- to 8-fold. This differential regulation coincided with the effects of these chemicals on the cornification of cultured TBE cells. Using MT5 as a probe, we have localized the tracheal spr1 gene on the human chromosome 1 by a Southern blot analysis using a panel of human-rodent somatic cell hybrid DNAs. The gene was further sublocalized to bands q22-23 by in situ hybridization.

Original languageEnglish (US)
Pages (from-to)104-111
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume7
Issue number1
StatePublished - Jul 1992
Externally publishedYes

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Chromosome Mapping
Proline
Epithelium
Genes
Epithelial Cells
Proteins
Complementary DNA
Clone Cells
Messenger RNA
Hybrid Cells
Chromosomes, Human, Pair 1
DNA sequences
Human Chromosomes
Phorbol Esters
Ultraviolet Rays
Chromosomes
Southern Blotting
Glutamine
Vitamin A
Gene Library

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

An unusual expression of a squamous cell marker, small proline-rich protein gene, in tracheobronchial epithelium : differential regulation and gene mapping. / An, G.; Huang, Hui-ming; Tesfaigzi, J.; Garcia-Heras, J.; Ledbetter, D. H.; Carlson, D. M.; Wu, R.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 7, No. 1, 07.1992, p. 104-111.

Research output: Contribution to journalArticle

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