An integration-free iPSC line, ICCSICi007-A, derived from a female Alzheimer's disease patient with the APOE-ε4/ε4 alleles

Eva Díaz-Guerra; Eva Rodríguez-Traver; Elena P. Moreno-Jiménez; Itziar de Rojas; César Rodríguez; María Orera; Isabel Hernández; Agustín Ruiz; Carlos Vicario

doi:10.1016/j.scr.2019.101588

An integration-free iPSC line, ICCSICi007-A, derived from a female Alzheimer's disease patient with the APOE-ε4/ε4 alleles

Eva Díaz-Guerra, Eva Rodríguez-Traver, Elena P. Moreno-Jiménez, Itziar de Rojas, César Rodríguez, María Orera, Isabel Hernández, Agustín Ruiz, Carlos Vicario

Research output: Contribution to journal › Article › peer-review

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Abstract

The epsilon4 (ε4) allele of the APOE gene, which encodes the apolipoprotein E4 (ApoE4), is the strongest genetic risk factor known for late-onset Alzheimer´s disease (LOAD). Here, we present the characterization of an iPSC line generated from dermal fibroblasts of a female AD patient using Sendai viral vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The iPSCs maintained the original genotype, a normal karyotype, were free from Sendai viral vectors and reprogramming factors, presented a normal morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency.

Original language	English (US)
Article number	101588
Journal	Stem Cell Research
Volume	41
DOIs	https://doi.org/10.1016/j.scr.2019.101588
State	Published - Dec 2019
Externally published	Yes

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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title = "An integration-free iPSC line, ICCSICi007-A, derived from a female Alzheimer's disease patient with the APOE-ε4/ε4 alleles",

abstract = "The epsilon4 (ε4) allele of the APOE gene, which encodes the apolipoprotein E4 (ApoE4), is the strongest genetic risk factor known for late-onset Alzheimer´s disease (LOAD). Here, we present the characterization of an iPSC line generated from dermal fibroblasts of a female AD patient using Sendai viral vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The iPSCs maintained the original genotype, a normal karyotype, were free from Sendai viral vectors and reprogramming factors, presented a normal morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency.",

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doi = "10.1016/j.scr.2019.101588",

language = "English (US)",

volume = "41",

journal = "Stem Cell Research",

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AU - Díaz-Guerra, Eva

AU - Rodríguez-Traver, Eva

AU - Moreno-Jiménez, Elena P.

AU - de Rojas, Itziar

AU - Rodríguez, César

AU - Orera, María

AU - Hernández, Isabel

AU - Ruiz, Agustín

AU - Vicario, Carlos

PY - 2019/12

Y1 - 2019/12

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AB - The epsilon4 (ε4) allele of the APOE gene, which encodes the apolipoprotein E4 (ApoE4), is the strongest genetic risk factor known for late-onset Alzheimer´s disease (LOAD). Here, we present the characterization of an iPSC line generated from dermal fibroblasts of a female AD patient using Sendai viral vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The iPSCs maintained the original genotype, a normal karyotype, were free from Sendai viral vectors and reprogramming factors, presented a normal morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency.

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An integration-free iPSC line, ICCSICi007-A, derived from a female Alzheimer's disease patient with the APOE-ε4/ε4 alleles

Abstract

ASJC Scopus subject areas

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