An Inhibitor of Trypsin-Like Activity in Rat Liver

Celia Kaye; Danica Dabich

doi:10.3181/00379727-131-34108

An Inhibitor of Trypsin-Like Activity in Rat Liver

Celia Kaye, Danica Dabich

Research output: Contribution to journal › Article › peer-review

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Abstract

Inhibitors of lysosomal and tryptic hydrolysis of benzoyl-dl-arginine-β-naphthylamide (BANA) were shown to be present in the microsomal and 100,000g supernatant fractions of rat liver homogenates. The 100,000g soluble inhibitor was shown to be: (1) capable of inhibiting tryptic digestion of casein and BANA; (2) insoluble at pH 4.0 but soluble at neutral or basic pH values; (3) nondialyzable; and (4) heat-stable. The inhibitor was maximally active at pH 7.4 and above during lysosomal hydrolysis of BANA.

Original language	English (US)
Pages (from-to)	1366-1368
Number of pages	3
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	131
Issue number	4
DOIs	https://doi.org/10.3181/00379727-131-34108
State	Published - Sep 1969
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "An Inhibitor of Trypsin-Like Activity in Rat Liver",

abstract = "Inhibitors of lysosomal and tryptic hydrolysis of benzoyl-dl-arginine-β-naphthylamide (BANA) were shown to be present in the microsomal and 100,000g supernatant fractions of rat liver homogenates. The 100,000g soluble inhibitor was shown to be: (1) capable of inhibiting tryptic digestion of casein and BANA; (2) insoluble at pH 4.0 but soluble at neutral or basic pH values; (3) nondialyzable; and (4) heat-stable. The inhibitor was maximally active at pH 7.4 and above during lysosomal hydrolysis of BANA.",

author = "Celia Kaye and Danica Dabich",

note = "Funding Information: These investigations were supported by funds from the Michigan Heart Association.",

year = "1969",

month = sep,

doi = "10.3181/00379727-131-34108",

language = "English (US)",

volume = "131",

pages = "1366--1368",

journal = "Proceedings of the Society for Experimental Biology and Medicine",

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T1 - An Inhibitor of Trypsin-Like Activity in Rat Liver

AU - Kaye, Celia

AU - Dabich, Danica

N1 - Funding Information: These investigations were supported by funds from the Michigan Heart Association.

PY - 1969/9

Y1 - 1969/9

N2 - Inhibitors of lysosomal and tryptic hydrolysis of benzoyl-dl-arginine-β-naphthylamide (BANA) were shown to be present in the microsomal and 100,000g supernatant fractions of rat liver homogenates. The 100,000g soluble inhibitor was shown to be: (1) capable of inhibiting tryptic digestion of casein and BANA; (2) insoluble at pH 4.0 but soluble at neutral or basic pH values; (3) nondialyzable; and (4) heat-stable. The inhibitor was maximally active at pH 7.4 and above during lysosomal hydrolysis of BANA.

AB - Inhibitors of lysosomal and tryptic hydrolysis of benzoyl-dl-arginine-β-naphthylamide (BANA) were shown to be present in the microsomal and 100,000g supernatant fractions of rat liver homogenates. The 100,000g soluble inhibitor was shown to be: (1) capable of inhibiting tryptic digestion of casein and BANA; (2) insoluble at pH 4.0 but soluble at neutral or basic pH values; (3) nondialyzable; and (4) heat-stable. The inhibitor was maximally active at pH 7.4 and above during lysosomal hydrolysis of BANA.

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JO - Proceedings of the Society for Experimental Biology and Medicine

JF - Proceedings of the Society for Experimental Biology and Medicine

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An Inhibitor of Trypsin-Like Activity in Rat Liver

Abstract

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