An infectious clone of woolly monkey hepatitis B virus

Robert E. Lanford, Deborah Chavez, Azeneth Barrera, Kathleen M. Brasky

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Members of the Hepadnaviridae family have been isolated from birds, rodents, and primates. A new hepadnavirus isolated from the woolly monkey, a New World primate, is phylogenetically distinct from other primate isolates. An animal model has been established for woolly monkey hepatitis B virus (WMHBV) by using spider monkeys, since woolly monkeys are endangered. In this study, a greater-than-genome length construct was prepared without amplification by using covalently closed circular DNA extracted from the liver of an infected woolly monkey. Transfection of the human liver cell line Huh7 with WMHBV DNA resulted in the production of viral transcripts, DNA replicative intermediates, and secreted virions at levels similar to those obtained with an infectious human HBV clone, demonstrating that the host range restriction of WMHBV is not at the level of genome replication. WMHBV particles from the medium of transfected cultures initiated an infection in a spider monkey similar to that obtained with virions derived from woolly monkey serum. In an attempt to adapt the virus for higher levels of replication in spider monkeys, immunosuppressed and newborn animals were inoculated. Neither procedure produced persistent infections, and the level of viral replication remained several logs lower than that observed in persistently infected woolly monkeys. These data demonstrate the production of an infectious clone for WMHBV and extend the characterization of the spider monkey animal model.

    Original languageEnglish (US)
    Pages (from-to)7814-7819
    Number of pages6
    JournalJournal of Virology
    Volume77
    Issue number14
    DOIs
    StatePublished - Jul 2003

    Fingerprint

    Woolly Monkey Sarcoma Virus
    Atelinae
    Cercopithecine Herpesvirus 1
    Hepatitis B virus
    monkeys
    Clone Cells
    clones
    Hepadnaviridae
    virion
    Araneae
    Primates
    Virion
    animal models
    circular DNA
    Cebidae
    Animal Models
    genome
    Genome
    DNA
    transfection

    ASJC Scopus subject areas

    • Immunology

    Cite this

    Lanford, R. E., Chavez, D., Barrera, A., & Brasky, K. M. (2003). An infectious clone of woolly monkey hepatitis B virus. Journal of Virology, 77(14), 7814-7819. https://doi.org/10.1128/JVI.77.14.7814-7819.2003

    An infectious clone of woolly monkey hepatitis B virus. / Lanford, Robert E.; Chavez, Deborah; Barrera, Azeneth; Brasky, Kathleen M.

    In: Journal of Virology, Vol. 77, No. 14, 07.2003, p. 7814-7819.

    Research output: Contribution to journalArticle

    Lanford, RE, Chavez, D, Barrera, A & Brasky, KM 2003, 'An infectious clone of woolly monkey hepatitis B virus', Journal of Virology, vol. 77, no. 14, pp. 7814-7819. https://doi.org/10.1128/JVI.77.14.7814-7819.2003
    Lanford, Robert E. ; Chavez, Deborah ; Barrera, Azeneth ; Brasky, Kathleen M. / An infectious clone of woolly monkey hepatitis B virus. In: Journal of Virology. 2003 ; Vol. 77, No. 14. pp. 7814-7819.
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