An in vitro assay of bone development using fetal long bones of mice: Morphological studies

Zvi Schwartz, A. Ornoy, W. A. Soskolne

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The purpose of this study was to examine the morphological changes in an in vitro system in which the two elements of bone modelling, formation and resorption, could be studied simultaneously. Pregnant mice were killed on days 15, 16 and 17 of gestation, the fetuses were removed and the radii and ulnae dissected free of soft tissue. The bones were cultured for 6 days in media (BGJ) supplemented with 20% fetal calf serum and 150 μg/ml vitamin C. Growth and mineralization were estimated by measuring the total length of the bone, and diaphysis, and by light transmission electron microscopy (TEM). The results of this study indicate that there is a continuous measurable increase in the total length of fetal mouse long bones over the 6 days of culture. These bones show a continuous growth of periosteal bone, with mesenchymal tissue penetrating into the diaphyseal shaft, and development of bone marrow like tissue. TEM examination showed differentiation of mesenchymal cells to osteoblasts, formation of new bone matrix and bone mineralization similar to that found in developmentally matched controls. In the cartilagenous epiphyses, however, many hydroxyapatite crystals were not associated with matrix vesicles. In addition, some of the chondrocytes of the hypertrophic zone appeared to be dedifferentiating into mesenchymal cells with osteoblast-like features. In spite of the lack of osteoclasts in the 15- and 16-day explants, osteoclasts appeared in the diaphysis after 2 and 4 days in culture. Our results suggest that this system can serve as a good model for the study of bone formation and resorption as they occur, simultaneously, during bone modelling. The 16-day-old explants would be the preferred bones to use over 15- or 17-day-old explants.

Original languageEnglish (US)
Pages (from-to)197-205
Number of pages9
JournalActa Anatomica
Volume124
Issue number3-4
StatePublished - 1985
Externally publishedYes

Fingerprint

Bone Development
Bone and Bones
Diaphyses
Osteoclasts
Bone Resorption
Osteoblasts
Transmission Electron Microscopy
Osteogenesis
Physiologic Calcification
Ulna
Epiphyses
Bone Matrix
Durapatite
Chondrocytes
In Vitro Techniques
Ascorbic Acid
Cell Differentiation
Fetus
Bone Marrow
Light

ASJC Scopus subject areas

  • Anatomy

Cite this

Schwartz, Z., Ornoy, A., & Soskolne, W. A. (1985). An in vitro assay of bone development using fetal long bones of mice: Morphological studies. Acta Anatomica, 124(3-4), 197-205.

An in vitro assay of bone development using fetal long bones of mice : Morphological studies. / Schwartz, Zvi; Ornoy, A.; Soskolne, W. A.

In: Acta Anatomica, Vol. 124, No. 3-4, 1985, p. 197-205.

Research output: Contribution to journalArticle

Schwartz, Z, Ornoy, A & Soskolne, WA 1985, 'An in vitro assay of bone development using fetal long bones of mice: Morphological studies', Acta Anatomica, vol. 124, no. 3-4, pp. 197-205.
Schwartz, Zvi ; Ornoy, A. ; Soskolne, W. A. / An in vitro assay of bone development using fetal long bones of mice : Morphological studies. In: Acta Anatomica. 1985 ; Vol. 124, No. 3-4. pp. 197-205.
@article{657ae2e867534dc6979fef4076864eb2,
title = "An in vitro assay of bone development using fetal long bones of mice: Morphological studies",
abstract = "The purpose of this study was to examine the morphological changes in an in vitro system in which the two elements of bone modelling, formation and resorption, could be studied simultaneously. Pregnant mice were killed on days 15, 16 and 17 of gestation, the fetuses were removed and the radii and ulnae dissected free of soft tissue. The bones were cultured for 6 days in media (BGJ) supplemented with 20{\%} fetal calf serum and 150 μg/ml vitamin C. Growth and mineralization were estimated by measuring the total length of the bone, and diaphysis, and by light transmission electron microscopy (TEM). The results of this study indicate that there is a continuous measurable increase in the total length of fetal mouse long bones over the 6 days of culture. These bones show a continuous growth of periosteal bone, with mesenchymal tissue penetrating into the diaphyseal shaft, and development of bone marrow like tissue. TEM examination showed differentiation of mesenchymal cells to osteoblasts, formation of new bone matrix and bone mineralization similar to that found in developmentally matched controls. In the cartilagenous epiphyses, however, many hydroxyapatite crystals were not associated with matrix vesicles. In addition, some of the chondrocytes of the hypertrophic zone appeared to be dedifferentiating into mesenchymal cells with osteoblast-like features. In spite of the lack of osteoclasts in the 15- and 16-day explants, osteoclasts appeared in the diaphysis after 2 and 4 days in culture. Our results suggest that this system can serve as a good model for the study of bone formation and resorption as they occur, simultaneously, during bone modelling. The 16-day-old explants would be the preferred bones to use over 15- or 17-day-old explants.",
author = "Zvi Schwartz and A. Ornoy and Soskolne, {W. A.}",
year = "1985",
language = "English (US)",
volume = "124",
pages = "197--205",
journal = "Cells Tissues Organs",
issn = "1422-6405",
publisher = "S. Karger AG",
number = "3-4",

}

TY - JOUR

T1 - An in vitro assay of bone development using fetal long bones of mice

T2 - Morphological studies

AU - Schwartz, Zvi

AU - Ornoy, A.

AU - Soskolne, W. A.

PY - 1985

Y1 - 1985

N2 - The purpose of this study was to examine the morphological changes in an in vitro system in which the two elements of bone modelling, formation and resorption, could be studied simultaneously. Pregnant mice were killed on days 15, 16 and 17 of gestation, the fetuses were removed and the radii and ulnae dissected free of soft tissue. The bones were cultured for 6 days in media (BGJ) supplemented with 20% fetal calf serum and 150 μg/ml vitamin C. Growth and mineralization were estimated by measuring the total length of the bone, and diaphysis, and by light transmission electron microscopy (TEM). The results of this study indicate that there is a continuous measurable increase in the total length of fetal mouse long bones over the 6 days of culture. These bones show a continuous growth of periosteal bone, with mesenchymal tissue penetrating into the diaphyseal shaft, and development of bone marrow like tissue. TEM examination showed differentiation of mesenchymal cells to osteoblasts, formation of new bone matrix and bone mineralization similar to that found in developmentally matched controls. In the cartilagenous epiphyses, however, many hydroxyapatite crystals were not associated with matrix vesicles. In addition, some of the chondrocytes of the hypertrophic zone appeared to be dedifferentiating into mesenchymal cells with osteoblast-like features. In spite of the lack of osteoclasts in the 15- and 16-day explants, osteoclasts appeared in the diaphysis after 2 and 4 days in culture. Our results suggest that this system can serve as a good model for the study of bone formation and resorption as they occur, simultaneously, during bone modelling. The 16-day-old explants would be the preferred bones to use over 15- or 17-day-old explants.

AB - The purpose of this study was to examine the morphological changes in an in vitro system in which the two elements of bone modelling, formation and resorption, could be studied simultaneously. Pregnant mice were killed on days 15, 16 and 17 of gestation, the fetuses were removed and the radii and ulnae dissected free of soft tissue. The bones were cultured for 6 days in media (BGJ) supplemented with 20% fetal calf serum and 150 μg/ml vitamin C. Growth and mineralization were estimated by measuring the total length of the bone, and diaphysis, and by light transmission electron microscopy (TEM). The results of this study indicate that there is a continuous measurable increase in the total length of fetal mouse long bones over the 6 days of culture. These bones show a continuous growth of periosteal bone, with mesenchymal tissue penetrating into the diaphyseal shaft, and development of bone marrow like tissue. TEM examination showed differentiation of mesenchymal cells to osteoblasts, formation of new bone matrix and bone mineralization similar to that found in developmentally matched controls. In the cartilagenous epiphyses, however, many hydroxyapatite crystals were not associated with matrix vesicles. In addition, some of the chondrocytes of the hypertrophic zone appeared to be dedifferentiating into mesenchymal cells with osteoblast-like features. In spite of the lack of osteoclasts in the 15- and 16-day explants, osteoclasts appeared in the diaphysis after 2 and 4 days in culture. Our results suggest that this system can serve as a good model for the study of bone formation and resorption as they occur, simultaneously, during bone modelling. The 16-day-old explants would be the preferred bones to use over 15- or 17-day-old explants.

UR - http://www.scopus.com/inward/record.url?scp=0022342102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022342102&partnerID=8YFLogxK

M3 - Article

C2 - 4082891

AN - SCOPUS:0022342102

VL - 124

SP - 197

EP - 205

JO - Cells Tissues Organs

JF - Cells Tissues Organs

SN - 1422-6405

IS - 3-4

ER -