An improved fixation procedure for microtubules and microfilaments in cells of the anterior pituitary gland

Jerzy B. Warchol, Damon C. Herbert, Edward G. Rennels

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A fixation procedure for the preservation of microtubules and microfilaments in anterior pituitary cells is presented. Small pieces of tissue were fixed at room temperature in a glutaraldehyde solution. The temperature was gradually reduced to 4–5°C before the tissue was post fixed. Post fixation was carried out using several changes of a mixture of cold glutaraldehyde and osmium tetroxide and then osmium tetroxide alone. The osmolarity of both fixing solutions was 400 milliosmoles. The procedure permits a clear visualization of both microtubules and microfilaments within anterior pituitary cells, particularly in regions where they are not ordinarily seen such as within the Golgi apparatus.

Original languageEnglish (US)
Pages (from-to)427-432
Number of pages6
JournalAmerican Journal of Anatomy
Volume141
Issue number3
DOIs
StatePublished - Nov 1974

ASJC Scopus subject areas

  • Anatomy

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