An HIV-1-infected T cell clone defective in IL-2 production and Ca2+ mobilization after CD3 stimulation

V. L. Perez, T. Rowe, J. S. Justement, S. T. Butera, C. H. June, T. M. Folks

    Research output: Contribution to journalArticle

    91 Scopus citations

    Abstract

    A chronically HIV-1-infected T cell clone (J1.1) derived from Jurkat cells was developed that possesses defects in CD3 signaling. This clone was phenotypically determined to be CD4- and express a reduced surface density of CD3 as compared with a pool of uninfected Jurkat clones. Although J1.1 could be induced with TNF-α to produce HIV-1 particles, stimulation via the CD3 (T3-Ti) complex, using mAb cross-linking, had no effect on viral production. Further investigation revealed that J1.1 secreted approximately 20-fold less IL-2 than did uninfected Jurkat cells after anti-CD3 treatment. In addition, a separate defect in Ca2+ mobilization was noted in the HIV-1-infected J1.1 line when compared with uninfected Jurkat cells after anti-CD3 cross-linking. The cell line described offers a new model in which to study the mechanisms of several defects directly imposed by HIV-1 on CD3+ cells.

    Original languageEnglish (US)
    Pages (from-to)3145-3148
    Number of pages4
    JournalJournal of Immunology
    Volume147
    Issue number9
    StatePublished - Jan 1 1991

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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    Perez, V. L., Rowe, T., Justement, J. S., Butera, S. T., June, C. H., & Folks, T. M. (1991). An HIV-1-infected T cell clone defective in IL-2 production and Ca2+ mobilization after CD3 stimulation. Journal of Immunology, 147(9), 3145-3148.