An F-box gene linked to the self-incompatibility (S) locus of Antirrhinum is expressed specifically in pollen and tapetum

Zhao Lai, Wenshi Ma, Bin Han, Lizhi Liang, Yansheng Zhang, Guofan Hong, Yongbiao Xue

Research output: Contribution to journalArticlepeer-review

263 Scopus citations

Abstract

In many flowering plants, self-fertilization is prevented by an intraspecific reproductive barrier known as self-incompatibility (SI), that, in most cases, is controlled by a single multiallelic S locus, So far, the only known S locus product in self-incompatible species from the Solanaceae, Scrophulariaceae and Rosaceae is a class of ribonucleases called S RNases. Molecular and transgenic analyses have shown that S RNases are responsible for pollen rejection by the pistil but have no role in pollen expression of SI, which appears to be mediated by a gene called the pollen self-incompatibility or Sp gene. To identify possible candidates for this gene, we investigated the genomic structure of the S locus in Antirrhinum, a member of the Scrophulariaceae. A novel F-box gene, AhSLF-S2, encoded by the S2 allele, with the expected features of the Sp gene was identified. AhSLF-S2 is located 9 kb downstream of S2 RNase gene and encodes a polypeptide of 376 amino acids with a conserved F-box domain in its amino-terminal part. Hypothetical genes homologous to AhSLF-S2 are apparent in the sequenced genomic DNA of Arabidopsis and rice. Together, they define a large gene family, named SLF (S locus F-box) family. AhSLF-S2 is highly polymorphic and is specifically expressed in tapetum, microspores and pollen grains in an allele-specific manner. The possibility that Sp encodes an F-box protein and the implications of this for the operation of self-incompatibility are discussed.

Original languageEnglish (US)
Pages (from-to)29-41
Number of pages13
JournalPlant Molecular Biology
Volume50
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Antirrhinum
  • F-box
  • Gametophyte
  • S locus structure
  • Self-incompatibility

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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