An epitope conserved in orthopoxvirus A13 envelope protein is the target of neutralizing and protective antibodies

Chungui Xu, Xiangzhi Meng, Bo Yan, Shane Crotty, Junpeng Deng, Yan Xiang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Primary immunization of humans with smallpox vaccine (live vaccinia virus (VACV)) consistently elicits antibody responses to six VACV virion membrane proteins, including A13. However, whether anti-A13 antibody contributes to immune protection against orthopoxviruses was unknown. Here, we isolated a murine monoclonal antibody (mAb) against A13 from a mouse that had been infected with VACV. The anti-A13 mAb bound to recombinant A13 protein with an affinity of 3.4. nM and neutralized VACV mature virions. Passive immunization of mice with the anti-A13 mAb protected against intranasal VACV infection. The epitope of the anti-A13 mAb was mapped to a 10-amino acid sequence conserved in all orthopoxviruses, including viriola virus and monkeypox virus, suggesting that anti-A13 antibodies elicited by smallpox vaccine might contribute to immune protection against orthopoxviruses. In addition, our data demonstrates that anti-A13 mAbs are effective for treating orthopoxvirus infection.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalVirology
Volume418
Issue number1
DOIs
StatePublished - Sep 15 2011

Fingerprint

Orthopoxvirus
Vaccinia virus
Neutralizing Antibodies
Epitopes
Monoclonal Antibodies
Smallpox Vaccine
Proteins
Virion
Anti-Idiotypic Antibodies
Monkeypox virus
Passive Immunization
Virus Diseases
Recombinant Proteins
Antibody Formation
Amino Acid Sequence
Immunization
Membrane Proteins
Viruses
Infection

Keywords

  • A13
  • Antibody
  • Poxvirus
  • Smallpox
  • Vaccine
  • Vaccinia

ASJC Scopus subject areas

  • Virology

Cite this

An epitope conserved in orthopoxvirus A13 envelope protein is the target of neutralizing and protective antibodies. / Xu, Chungui; Meng, Xiangzhi; Yan, Bo; Crotty, Shane; Deng, Junpeng; Xiang, Yan.

In: Virology, Vol. 418, No. 1, 15.09.2011, p. 67-73.

Research output: Contribution to journalArticle

Xu, Chungui ; Meng, Xiangzhi ; Yan, Bo ; Crotty, Shane ; Deng, Junpeng ; Xiang, Yan. / An epitope conserved in orthopoxvirus A13 envelope protein is the target of neutralizing and protective antibodies. In: Virology. 2011 ; Vol. 418, No. 1. pp. 67-73.
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