An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae

J. Morrison-Plummer, D. H. Jones, J. B. Baseman

Research output: Contribution to journalArticle

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for analysis of monoclonal antibodies directed towards lipid determinants of Mycoplasma pneumoniae. Chloroform-methanol lipid extracts as well as chromatographed lipid fractions of M. pneumoniae were bound to polyvinylchloride microtiter cells. Of 293 clones positive to M. pneumoniae as detected by a whole cell ELISA, a total of 78 clones produced antibodies which selectively bound to lipid extracts of M. pneumoniae. The simplicity and reproducibility of the assay permit rapid screening for detection of antibodies directed against non-protein cellular antigens.

Original languageEnglish (US)
Pages (from-to)165-178
Number of pages14
JournalJournal of Immunological Methods
Volume64
Issue number1-2
DOIs
StatePublished - Nov 11 1983

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Mycoplasma pneumoniae
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Lipids
Clone Cells
Antibodies
Chloroform
Methanol
Antigens

Keywords

  • ELISA
  • lipids
  • monoclonal antibody
  • Mycoplasma pneumoniae

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae. / Morrison-Plummer, J.; Jones, D. H.; Baseman, J. B.

In: Journal of Immunological Methods, Vol. 64, No. 1-2, 11.11.1983, p. 165-178.

Research output: Contribution to journalArticle

Morrison-Plummer, J. ; Jones, D. H. ; Baseman, J. B. / An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae. In: Journal of Immunological Methods. 1983 ; Vol. 64, No. 1-2. pp. 165-178.
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