An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae

J. Morrison-Plummer, D. H. Jones, J. B. Baseman

Research output: Contribution to journalArticlepeer-review

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for analysis of monoclonal antibodies directed towards lipid determinants of Mycoplasma pneumoniae. Chloroform-methanol lipid extracts as well as chromatographed lipid fractions of M. pneumoniae were bound to polyvinylchloride microtiter cells. Of 293 clones positive to M. pneumoniae as detected by a whole cell ELISA, a total of 78 clones produced antibodies which selectively bound to lipid extracts of M. pneumoniae. The simplicity and reproducibility of the assay permit rapid screening for detection of antibodies directed against non-protein cellular antigens.

Original languageEnglish (US)
Pages (from-to)165-178
Number of pages14
JournalJournal of Immunological Methods
Volume64
Issue number1-2
DOIs
StatePublished - Nov 11 1983

Keywords

  • ELISA
  • Mycoplasma pneumoniae
  • lipids
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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