An efficient method for routine epstein-barr virus immortalization of human B lymphocytes

Florence E. Wall, Richard D. Henkel, Michael P. Stern, Hal B. Jenson, Mary Pat Moyer

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

A variety of methods exist for the immortalization of B lymphocytes by Epstein-Barr virus due to the simplicity of such techniques to establish cell lines with stable genomic DNA. Two different methods for immortalizing lymphoblastoid cell lines were compared for differences in techniques and materials, time between initiation and immortalization, and success rate of immortalization. An incubation period in Epstein-Barr virus and the use of conditioned media improved immortalization efficiency from 86 to 98% and decreased the time (usually weeks) from culture initiation to cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in non-insulin-dependent diabetes mellitus.

Original languageEnglish (US)
Pages (from-to)156-159
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume31
Issue number2
DOIs
StatePublished - Feb 1995

Keywords

  • B lymphocytes
  • Epstein-Barr virus
  • human cell lines
  • immortalization
  • transformation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'An efficient method for routine epstein-barr virus immortalization of human B lymphocytes'. Together they form a unique fingerprint.

Cite this