An efficient method for routine epstein-barr virus immortalization of human B lymphocytes

Florence E. Wall; Richard D. Henkel; Michael P. Stern; Hal B. Jenson; Mary Pat Moyer

doi:10.1007/BF02633976

An efficient method for routine epstein-barr virus immortalization of human B lymphocytes

Florence E. Wall, Richard D. Henkel, Michael P. Stern, Hal B. Jenson, Mary Pat Moyer

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

A variety of methods exist for the immortalization of B lymphocytes by Epstein-Barr virus due to the simplicity of such techniques to establish cell lines with stable genomic DNA. Two different methods for immortalizing lymphoblastoid cell lines were compared for differences in techniques and materials, time between initiation and immortalization, and success rate of immortalization. An incubation period in Epstein-Barr virus and the use of conditioned media improved immortalization efficiency from 86 to 98% and decreased the time (usually weeks) from culture initiation to cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in non-insulin-dependent diabetes mellitus.

Original language	English (US)
Pages (from-to)	156-159
Number of pages	4
Journal	In Vitro Cellular and Developmental Biology - Plant
Volume	31
Issue number	2
DOIs	https://doi.org/10.1007/BF02633976
State	Published - Feb 1995

Keywords

B lymphocytes
Epstein-Barr virus
human cell lines
immortalization
transformation

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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10.1007/BF02633976

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title = "An efficient method for routine epstein-barr virus immortalization of human B lymphocytes",

abstract = "A variety of methods exist for the immortalization of B lymphocytes by Epstein-Barr virus due to the simplicity of such techniques to establish cell lines with stable genomic DNA. Two different methods for immortalizing lymphoblastoid cell lines were compared for differences in techniques and materials, time between initiation and immortalization, and success rate of immortalization. An incubation period in Epstein-Barr virus and the use of conditioned media improved immortalization efficiency from 86 to 98% and decreased the time (usually weeks) from culture initiation to cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in non-insulin-dependent diabetes mellitus.",

keywords = "B lymphocytes, Epstein-Barr virus, human cell lines, immortalization, transformation",

author = "Wall, {Florence E.} and Henkel, {Richard D.} and Stern, {Michael P.} and Jenson, {Hal B.} and Moyer, {Mary Pat}",

year = "1995",

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T1 - An efficient method for routine epstein-barr virus immortalization of human B lymphocytes

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AU - Henkel, Richard D.

AU - Stern, Michael P.

AU - Jenson, Hal B.

AU - Moyer, Mary Pat

PY - 1995/2

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AB - A variety of methods exist for the immortalization of B lymphocytes by Epstein-Barr virus due to the simplicity of such techniques to establish cell lines with stable genomic DNA. Two different methods for immortalizing lymphoblastoid cell lines were compared for differences in techniques and materials, time between initiation and immortalization, and success rate of immortalization. An incubation period in Epstein-Barr virus and the use of conditioned media improved immortalization efficiency from 86 to 98% and decreased the time (usually weeks) from culture initiation to cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in non-insulin-dependent diabetes mellitus.

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KW - immortalization

KW - transformation

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An efficient method for routine epstein-barr virus immortalization of human B lymphocytes

Abstract

Keywords

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