Abstract
Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2d presenter cells for interleukin 2 (IL-2) production. The autoreactive clone showed helper activity only in the presence of suboptimal numbers of antigen (Ag)-primed T cells. Both T hybridomas were Lyt 1+2+ and Thy 1+. Cells produced from such fusions should provide a useful instrument not only in dissecting the T-cell regulatory mechanism, but also in isolating and characterizing self-recognition structures.
Original language | English (US) |
---|---|
Pages (from-to) | 183-189 |
Number of pages | 7 |
Journal | Cellular Immunology |
Volume | 86 |
Issue number | 1 |
DOIs | |
State | Published - Jun 1984 |
ASJC Scopus subject areas
- Immunology