Abstract
Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2d presenter cells for interleukin 2 (IL-2) production. The autoreactive clone showed helper activity only in the presence of suboptimal numbers of antigen (Ag)-primed T cells. Both T hybridomas were Lyt 1+2+ and Thy 1+. Cells produced from such fusions should provide a useful instrument not only in dissecting the T-cell regulatory mechanism, but also in isolating and characterizing self-recognition structures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 183-189 |
| Number of pages | 7 |
| Journal | Cellular Immunology |
| Volume | 86 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 1984 |
ASJC Scopus subject areas
- Immunology
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Folks, T. M., & Sell, K. W. (1984). An autoreactive T hybridoma expressing nonspecific helper activity. Cellular Immunology, 86(1), 183-189. https://doi.org/10.1016/0008-8749(84)90371-X