An autoreactive T hybridoma expressing nonspecific helper activity

Thomas M. Folks; Kenneth W. Sell

doi:10.1016/0008-8749(84)90371-X

An autoreactive T hybridoma expressing nonspecific helper activity

Thomas M. Folks, Kenneth W. Sell

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2^d presenter cells for interleukin 2 (IL-2) production. The autoreactive clone showed helper activity only in the presence of suboptimal numbers of antigen (Ag)-primed T cells. Both T hybridomas were Lyt 1⁺2⁺ and Thy 1⁺. Cells produced from such fusions should provide a useful instrument not only in dissecting the T-cell regulatory mechanism, but also in isolating and characterizing self-recognition structures.

Original language	English (US)
Pages (from-to)	183-189
Number of pages	7
Journal	Cellular Immunology
Volume	86
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(84)90371-X
State	Published - Jun 1984

ASJC Scopus subject areas

Immunology

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title = "An autoreactive T hybridoma expressing nonspecific helper activity",

abstract = "Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2d presenter cells for interleukin 2 (IL-2) production. The autoreactive clone showed helper activity only in the presence of suboptimal numbers of antigen (Ag)-primed T cells. Both T hybridomas were Lyt 1+2+ and Thy 1+. Cells produced from such fusions should provide a useful instrument not only in dissecting the T-cell regulatory mechanism, but also in isolating and characterizing self-recognition structures.",

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AU - Sell, Kenneth W.

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AB - Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2d presenter cells for interleukin 2 (IL-2) production. The autoreactive clone showed helper activity only in the presence of suboptimal numbers of antigen (Ag)-primed T cells. Both T hybridomas were Lyt 1+2+ and Thy 1+. Cells produced from such fusions should provide a useful instrument not only in dissecting the T-cell regulatory mechanism, but also in isolating and characterizing self-recognition structures.

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