An autoreactive T hybridoma expressing nonspecific helper activity

Thomas M. Folks, Kenneth W. Sell

    Research output: Contribution to journalArticle

    2 Scopus citations

    Abstract

    Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2d presenter cells for interleukin 2 (IL-2) production. The autoreactive clone showed helper activity only in the presence of suboptimal numbers of antigen (Ag)-primed T cells. Both T hybridomas were Lyt 1+2+ and Thy 1+. Cells produced from such fusions should provide a useful instrument not only in dissecting the T-cell regulatory mechanism, but also in isolating and characterizing self-recognition structures.

    Original languageEnglish (US)
    Pages (from-to)183-189
    Number of pages7
    JournalCellular Immunology
    Volume86
    Issue number1
    DOIs
    StatePublished - Jun 1984

    ASJC Scopus subject areas

    • Immunology

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