Amphiphilic quaternary ammonium chitosan/sodium alginate multilayer coatings kill fungal cells and inhibit fungal biofilm on dental biomaterials

Joonhoo Jung, Lin Li, Chih-ko Yeh, Xuehong Ren, Yuyu Sun

Research output: Contribution to journalArticle

Abstract

Formation of fungal biofilms on health care-related materials causes serious clinical consequences. This study reports a novel fungal repelling strategy to control fungal biofilm formation on denture biomaterials through layer-by-layer self-assembly (LBL). Amphiphilic quaternary ammonium chitosans (CS612) were synthesized and used as the antimicrobial positive layer, and sodium alginate (SA) was chosen as the negative layer to construct LBL multilayers on poly (methyl methacrylate) (PMMA)-based denture materials. The presence of LBL multilayers on denture disc was confirmed and characterized by surface zeta potential, water contact angle, AFM, and FT-IR analyses. The multilayer coatings, especially CS612 as the outmost layer, effectively prevented the fungal initial adhesion and biofilm formation. The Candida cells avoided the multilayer coatings and suspended in broth solution instead of forming biofilms, suggesting that the LBL multilayers had fungal repelling effects. The LBL multilayers were biocompatible toward mammalian cells. In stability tests, after immersion in PBS for 4 weeks under constant shaking and repeated brushing with a denture brush for up to 3000 times, the biofilm-controlling effects of the LBL multilayers were not affected, pointing to a novel long-term strategy in controlling fungal biofilms on denture and other related biomaterials.

Original languageEnglish (US)
Article number109961
JournalMaterials Science and Engineering C
Volume104
DOIs
StatePublished - Nov 1 2019

Fingerprint

Sodium alginate
biofilms
Chitosan
Biofilms
Biocompatible Materials
Ammonium Compounds
Biomaterials
Dental prostheses
Multilayers
sodium
coatings
Coatings
cells
broths
stability tests
shaking
brushes
Candida
polymethyl methacrylate
submerging

Keywords

  • Antimicrobial multilayer
  • Biocompatible
  • Fungal repellent
  • Layer-by-layer
  • Quaternary ammonium chitosan

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Amphiphilic quaternary ammonium chitosan/sodium alginate multilayer coatings kill fungal cells and inhibit fungal biofilm on dental biomaterials. / Jung, Joonhoo; Li, Lin; Yeh, Chih-ko; Ren, Xuehong; Sun, Yuyu.

In: Materials Science and Engineering C, Vol. 104, 109961, 01.11.2019.

Research output: Contribution to journalArticle

@article{58d418c1ebc244f7ab1858fd6ddd80a3,
title = "Amphiphilic quaternary ammonium chitosan/sodium alginate multilayer coatings kill fungal cells and inhibit fungal biofilm on dental biomaterials",
abstract = "Formation of fungal biofilms on health care-related materials causes serious clinical consequences. This study reports a novel fungal repelling strategy to control fungal biofilm formation on denture biomaterials through layer-by-layer self-assembly (LBL). Amphiphilic quaternary ammonium chitosans (CS612) were synthesized and used as the antimicrobial positive layer, and sodium alginate (SA) was chosen as the negative layer to construct LBL multilayers on poly (methyl methacrylate) (PMMA)-based denture materials. The presence of LBL multilayers on denture disc was confirmed and characterized by surface zeta potential, water contact angle, AFM, and FT-IR analyses. The multilayer coatings, especially CS612 as the outmost layer, effectively prevented the fungal initial adhesion and biofilm formation. The Candida cells avoided the multilayer coatings and suspended in broth solution instead of forming biofilms, suggesting that the LBL multilayers had fungal repelling effects. The LBL multilayers were biocompatible toward mammalian cells. In stability tests, after immersion in PBS for 4 weeks under constant shaking and repeated brushing with a denture brush for up to 3000 times, the biofilm-controlling effects of the LBL multilayers were not affected, pointing to a novel long-term strategy in controlling fungal biofilms on denture and other related biomaterials.",
keywords = "Antimicrobial multilayer, Biocompatible, Fungal repellent, Layer-by-layer, Quaternary ammonium chitosan",
author = "Joonhoo Jung and Lin Li and Chih-ko Yeh and Xuehong Ren and Yuyu Sun",
year = "2019",
month = "11",
day = "1",
doi = "10.1016/j.msec.2019.109961",
language = "English (US)",
volume = "104",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Amphiphilic quaternary ammonium chitosan/sodium alginate multilayer coatings kill fungal cells and inhibit fungal biofilm on dental biomaterials

AU - Jung, Joonhoo

AU - Li, Lin

AU - Yeh, Chih-ko

AU - Ren, Xuehong

AU - Sun, Yuyu

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Formation of fungal biofilms on health care-related materials causes serious clinical consequences. This study reports a novel fungal repelling strategy to control fungal biofilm formation on denture biomaterials through layer-by-layer self-assembly (LBL). Amphiphilic quaternary ammonium chitosans (CS612) were synthesized and used as the antimicrobial positive layer, and sodium alginate (SA) was chosen as the negative layer to construct LBL multilayers on poly (methyl methacrylate) (PMMA)-based denture materials. The presence of LBL multilayers on denture disc was confirmed and characterized by surface zeta potential, water contact angle, AFM, and FT-IR analyses. The multilayer coatings, especially CS612 as the outmost layer, effectively prevented the fungal initial adhesion and biofilm formation. The Candida cells avoided the multilayer coatings and suspended in broth solution instead of forming biofilms, suggesting that the LBL multilayers had fungal repelling effects. The LBL multilayers were biocompatible toward mammalian cells. In stability tests, after immersion in PBS for 4 weeks under constant shaking and repeated brushing with a denture brush for up to 3000 times, the biofilm-controlling effects of the LBL multilayers were not affected, pointing to a novel long-term strategy in controlling fungal biofilms on denture and other related biomaterials.

AB - Formation of fungal biofilms on health care-related materials causes serious clinical consequences. This study reports a novel fungal repelling strategy to control fungal biofilm formation on denture biomaterials through layer-by-layer self-assembly (LBL). Amphiphilic quaternary ammonium chitosans (CS612) were synthesized and used as the antimicrobial positive layer, and sodium alginate (SA) was chosen as the negative layer to construct LBL multilayers on poly (methyl methacrylate) (PMMA)-based denture materials. The presence of LBL multilayers on denture disc was confirmed and characterized by surface zeta potential, water contact angle, AFM, and FT-IR analyses. The multilayer coatings, especially CS612 as the outmost layer, effectively prevented the fungal initial adhesion and biofilm formation. The Candida cells avoided the multilayer coatings and suspended in broth solution instead of forming biofilms, suggesting that the LBL multilayers had fungal repelling effects. The LBL multilayers were biocompatible toward mammalian cells. In stability tests, after immersion in PBS for 4 weeks under constant shaking and repeated brushing with a denture brush for up to 3000 times, the biofilm-controlling effects of the LBL multilayers were not affected, pointing to a novel long-term strategy in controlling fungal biofilms on denture and other related biomaterials.

KW - Antimicrobial multilayer

KW - Biocompatible

KW - Fungal repellent

KW - Layer-by-layer

KW - Quaternary ammonium chitosan

UR - http://www.scopus.com/inward/record.url?scp=85068543595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068543595&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2019.109961

DO - 10.1016/j.msec.2019.109961

M3 - Article

VL - 104

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

M1 - 109961

ER -